Asking B, Gjörstrup P
Acta Physiol Scand. 1987 Jul;130(3):439-45. doi: 10.1111/j.1748-1716.1987.tb08160.x.
Parasympathetic and sympathetic nerve stimulation in vivo, individually and in combination, was used to study secretion and synthesis of amylase in the rat parotid gland. After 30 min with sympathetic nerve stimulation (3 Hz) a decrease in glandular amylase was seen, which corresponded approximately to the salivary output. On the other hand, after parasympathetic stimulation (10 Hz), chosen to obtain comparable amylase output, there was no decrease in glandular amylase, which points to synthesis during such activation. Experiments with incorporation of [3H]leucine, reflecting amylase synthesis, showed that both types of nerve stimulations increased such uptake in parotid protein. These results indicate that beside sympathetic activity, which is the main stimulus for granular amylase secretion, parasympathetic nerve impulses can evoke considerable amylase secretion because amylase synthesis is stimulated and amylase is rapidly available from a special, possibly non-granular pool. As expected from previous experiments an augmentation of amylase secretion was found, and the present experiments also indicated an augmentation at the level of synthesis when the two nerves were stimulated at the same time.
在体内分别或联合使用副交感神经和交感神经刺激，以研究大鼠腮腺中淀粉酶的分泌和合成。交感神经刺激（3Hz）30分钟后，腺体内淀粉酶减少，这与唾液分泌量大致相当。另一方面，选择副交感神经刺激（10Hz）以获得相当的淀粉酶分泌量，此时腺体内淀粉酶没有减少，这表明在此种激活过程中有合成作用。用[3H]亮氨酸掺入实验反映淀粉酶合成，结果表明两种类型的神经刺激均增加腮腺蛋白对其摄取。这些结果表明，除了作为颗粒性淀粉酶分泌主要刺激因素的交感神经活动外，副交感神经冲动也可引起相当量的淀粉酶分泌，因为淀粉酶合成受到刺激，且淀粉酶可从一个特殊的、可能是非颗粒性的储备池中迅速获取。正如先前实验所预期的那样，发现淀粉酶分泌增加，并且本实验还表明，当同时刺激两条神经时，在合成水平上也有增加。
