Experimental approaches to a rapid ethanol fermentation of glucose by aZymomonas mobilis strain.

Rapid ethanol fermentation is defined as a fermentation in which the ethanol content increases from 0 to 94.8 g 1(-1) in 6 horless. To achieve this by the fermentation of glucose withZymomonas mobilis, the initial biomass concentration must be at least 20 g dry wt 1(-1) and that of the substrate must not exceed 150 to 200 g 1(-1) during fermentation. The best results were obtained with a medium containing initielly 16% of the total sugar with the remaining glucose being added continuously, after 20 min of incubation, over 5 h at a rate of 0.21 g/min. After 6 h, ethanol reached 102 g 1(-1), the volumetric productivity was 17g ethanol 1(-1) h(-1) and the yield 79.8 or 88% of the theoretical value, calculated according to the total fed or the consumed glucose, respectively.