Yeger H, Baumal R, Harason P, Phillips M J
Am J Clin Pathol. 1987 Sep;88(3):278-85. doi: 10.1093/ajcp/88.3.278.
The lectin histochemical staining patterns of nine surgically resected Wilms' tumors (WIT) (five classical, one rhabdomyomatous, one monomorphic tubular, and two blastematous) and four WIT heterotransplants in nude mice were compared with those of six normal adult and fetal kidneys using 11 biotinylated and fluorochrome-labeled lectins representing a spectrum of sugar specificities. Tubular epithelial cells of fetal and adult kidney demonstrated a complex pattern of glycosubstances, as detected by staining with most of the lectins. The dysplastic tubules of WIT and WIT heterotransplants resembled distal convoluted tubules and collecting ducts of adult kidney, as detected by staining with lectins that reacted exclusively with these portions of the nephron. A simpler lectin affinity profile was observed with blastema of normal fetal kidney and blastema of WIT and WIT heterotransplants. Thus, fetal, adult, and tumor tubules and fetal and tumor blastema resemble each other in terms of lectin binding patterns.
使用11种代表一系列糖特异性的生物素化和荧光染料标记的凝集素，将9例手术切除的肾母细胞瘤（WIT）（5例经典型、1例横纹肌瘤型、1例单形管状型和2例胚芽型）及4例在裸鼠体内的WIT异种移植瘤的凝集素组织化学染色模式，与6例正常成人及胎儿肾脏的染色模式进行比较。胎儿和成人肾脏的肾小管上皮细胞经大多数凝集素染色后，显示出复杂的糖物质模式。WIT及WIT异种移植瘤的发育异常肾小管类似于成人肾脏的远曲小管和集合管，这是通过用仅与肾单位这些部分发生反应的凝集素染色检测到的。在正常胎儿肾脏的胚芽组织以及WIT和WIT异种移植瘤的胚芽组织中观察到更简单的凝集素亲和力图谱。因此，胎儿、成人及肿瘤的肾小管以及胎儿和肿瘤的胚芽组织在凝集素结合模式方面彼此相似。
