通过多位点聚合酶链反应结合电喷雾电离质谱法快速鉴定腹膜透析相关腹膜炎患者腹膜透析流出液中的细菌和念珠菌病原体。
Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.
作者信息
Chang Yu-Tzu, Wang Hsuan-Chen, Wang Ming-Cheng, Wu An-Bang, Sung Junne-Ming, Sun H Sunny, Su Ih-Jen, Kan Wei-Chih, Chien Chih-Chiang, Hwang Jyh-Chang, Wang Hsien-Yi, Tseng Chin-Chung, Wu Chi-Jung
机构信息
Department of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan.
出版信息
J Clin Microbiol. 2014 Apr;52(4):1217-9. doi: 10.1128/JCM.03106-13. Epub 2014 Jan 15.
Abstract
PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
摘要
将聚合酶链反应（PCR）与电喷雾电离质谱法（PCR/ESI-MS）相结合，用于检测腹膜透析（PD）相关性腹膜炎的病原体，并与培养法进行比较。在21份PD流出液样本中，PCR/ESI-MS在18份（86%）样本中鉴定出微生物，其中1份培养阴性样本中检测出结核分枝杆菌。在15份双阳性样本中，PCR/ESI-MS与培养法在属水平和种水平的一致性分别达到100%（15/15）和87.5%（7/8）。PCR/ESI-MS可用于快速检测PD相关性腹膜炎的病原体。
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