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本文引用的文献

1
Enhanced diagnostic yields of bacteremia and candidemia in blood specimens by PCR-electrospray ionization mass spectrometry.PCR-电喷雾电离质谱法增强血培养标本中菌血症和念珠菌血症的诊断产量。
J Clin Microbiol. 2013 Nov;51(11):3535-41. doi: 10.1128/JCM.00876-13. Epub 2013 Aug 21.
2
Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles.使用 PCR 结合电喷雾电离质谱技术从血培养瓶中快速鉴定细菌和酵母血流感染病原体。
J Clin Microbiol. 2011 Jan;49(1):345-53. doi: 10.1128/JCM.00936-10. Epub 2010 Nov 3.
3
Peritoneal dialysis-related infections recommendations: 2010 update.腹膜透析相关感染的建议:2010年更新版
Perit Dial Int. 2010 Jul-Aug;30(4):393-423. doi: 10.3747/pdi.2010.00049.
4
Polymicrobial peritonitis in peritoneal dialysis patients in Australia: predictors, treatment, and outcomes.澳大利亚腹膜透析患者的多微生物性腹膜炎:预测因素、治疗和结局。
Am J Kidney Dis. 2010 Jan;55(1):121-31. doi: 10.1053/j.ajkd.2009.08.020. Epub 2009 Nov 22.
5
Analytical comparison of nine PCR primer sets designed to detect the presence of Escherichia coli/Shigella in water samples.用于检测水样中大肠杆菌/志贺氏菌的九种PCR引物组的分析比较。
Water Res. 2009 Jul;43(12):3019-28. doi: 10.1016/j.watres.2009.04.017. Epub 2009 Apr 23.
6
Identification of species of Abiotrophia, Enterococcus, Granulicatella and Streptococcus by sequence analysis of the ribosomal 16S-23S intergenic spacer region.通过核糖体16S - 23S基因间隔区序列分析鉴定阿托菲氏菌属、肠球菌属、颗粒链菌属和链球菌属的菌种
J Med Microbiol. 2007 Apr;56(Pt 4):504-513. doi: 10.1099/jmm.0.47027-0.
7
Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis.通过聚合酶链反应扩增23S rRNA在检测连续性非卧床腹膜透析腹膜炎细菌中的应用价值。
Am J Nephrol. 2006;26(2):115-20. doi: 10.1159/000092040. Epub 2006 Mar 14.
8
Comparative study of diagnosis of PD peritonitis by quantitative polymerase chain reaction for bacterial DNA vs culture methods.通过细菌DNA定量聚合酶链反应与培养方法诊断腹膜透析相关性腹膜炎的比较研究
J Nephrol. 2006 Jan-Feb;19(1):45-9.
9
Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing.通过16S rRNA、hsp65、sodA、recA和rpoB基因测序剖析19种快速生长分枝杆菌物种之间的系统发育关系。
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2095-2105. doi: 10.1099/ijs.0.63094-0.
10
PCR detection and identification of oral streptococci in saliva samples using gtf genes.利用gtf基因对唾液样本中的口腔链球菌进行PCR检测与鉴定。
Diagn Microbiol Infect Dis. 2004 Mar;48(3):195-9. doi: 10.1016/j.diagmicrobio.2003.10.002.

通过多位点聚合酶链反应结合电喷雾电离质谱法快速鉴定腹膜透析相关腹膜炎患者腹膜透析流出液中的细菌和念珠菌病原体。

Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.

作者信息

Chang Yu-Tzu, Wang Hsuan-Chen, Wang Ming-Cheng, Wu An-Bang, Sung Junne-Ming, Sun H Sunny, Su Ih-Jen, Kan Wei-Chih, Chien Chih-Chiang, Hwang Jyh-Chang, Wang Hsien-Yi, Tseng Chin-Chung, Wu Chi-Jung

机构信息

Department of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan.

出版信息

J Clin Microbiol. 2014 Apr;52(4):1217-9. doi: 10.1128/JCM.03106-13. Epub 2014 Jan 15.

DOI:10.1128/JCM.03106-13
PMID:24430451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3993497/
Abstract

PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

摘要

将聚合酶链反应(PCR)与电喷雾电离质谱法(PCR/ESI-MS)相结合,用于检测腹膜透析(PD)相关性腹膜炎的病原体,并与培养法进行比较。在21份PD流出液样本中,PCR/ESI-MS在18份(86%)样本中鉴定出微生物,其中1份培养阴性样本中检测出结核分枝杆菌。在15份双阳性样本中,PCR/ESI-MS与培养法在属水平和种水平的一致性分别达到100%(15/15)和87.5%(7/8)。PCR/ESI-MS可用于快速检测PD相关性腹膜炎的病原体。