Wildes R A, Pitman M G, Schaefer N
School of Biological Sciences, University of Sydney, Sydney, N.S.W., Australia.
Planta. 1976 Jan;128(1):35-40. doi: 10.1007/BF00397176.
Cycloheximide was shown to inhibit the transport of ions into the xylem of "salt-saturated" barley roots (Hordeum vulgare cv cape), and incorporation of L-[1-(14)C] leucine into protein within 40-60 min. Water flow across the roots of whole seedlings was not altered for at least 180 min. Uptake of ions into the cells of "salt-saturated" roots was not affected for 120 min, but was inhibited when treated with cycloheximide for more than 120 min. H(+) efflux from "lowsalt" roots was inhibited by cycloheximide at about the same time as ion uptake. Measurement of ATP levels and O2uptake indicated that cycloheximide was not acting as an uncoupler of oxidative phosphorylation. The present data are considered to support the view that secretion of ions into the xylem vessels involves a specific protein with a short effective half-life, and is a separate process from active uptake into the cortical cells.
已表明放线菌酮可抑制离子向“盐饱和”大麦根（大麦品种开普）木质部的运输，并在40 - 60分钟内抑制L-[1-(14)C]亮氨酸掺入蛋白质。至少180分钟内，全株幼苗根的水流未发生改变。120分钟内，“盐饱和”根细胞对离子的吸收不受影响，但用放线菌酮处理超过120分钟时会受到抑制。“低盐”根的H(+)外流在离子吸收的大致相同时间受到放线菌酮抑制。ATP水平和O2吸收的测量表明，放线菌酮并非作为氧化磷酸化的解偶联剂起作用。目前的数据被认为支持以下观点：离子向木质部导管的分泌涉及一种有效半衰期短的特定蛋白质，并且是一个与向皮层细胞的主动吸收相分离的过程。
