Institute of Agricultural Chemistry, University of Padua, Padova Italy.
Plant Physiol. 1975 Oct;56(4):478-80. doi: 10.1104/pp.56.4.478.
Inhibition by cycloheximide of sulfate transport in both barley roots (Hordeum vulgare L.) and potato tuber (Solanum tuberosum L.) increases with increasing inhibitor concentration only to a limited extent, depending on the length of the tissue incubation with the inhibitor. In contrast to this, increasing concentrations of dinitrophenol have a rapid and total inhibitory effect on the active transport. Leucine transport in the same tissues is strongly inhibited by dinitrophenol but is not affected by cycloheximide, whereas incorporation into protein is mainly inhibited by cycloheximide. It appears that the mechanism of transport inhibition by cycloheximide in plant tissues consists in stopping new carrier synthesis and not in the disruption of energy flow. Sulfate carriers show comparable decay rates in barley roots and potato tuber, the mean life being shorter than that of the leucine carriers. These appear more stable in roots than in storage tissues.
环己亚胺对大麦根（Hordeum vulgare L.）和马铃薯块茎（Solanum tuberosum L.）硫酸盐转运的抑制作用随抑制剂浓度的增加而增加，但仅限于一定程度，这取决于组织与抑制剂孵育的时间长短。与此相反，二硝基苯酚浓度的增加对主动转运有快速和完全的抑制作用。在相同的组织中，亮氨酸转运被二硝基苯酚强烈抑制，但不受环己亚胺影响，而掺入蛋白质主要受环己亚胺抑制。看来，环己亚胺在植物组织中抑制转运的机制在于阻止新载体的合成，而不是破坏能量流。硫酸盐载体在大麦根和马铃薯块茎中表现出相似的衰减率，平均寿命短于亮氨酸载体。这些在根中比在储存组织中更稳定。
