Department of Otolaryngology, Charles R. Drew University of Medicine and Science, Los Angeles, CA 90059, USA.
Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), California Nanosystems Institute at UCLA, Los Angeles, CA 90095, USA.
Int J Oncol. 2014 Mar;44(3):830-7. doi: 10.3892/ijo.2014.2258. Epub 2014 Jan 15.
We examined the apoptotic effect of a novel Probiotics Fermentation Technology (PFT) kefir grain product; PFT is a natural mixture composed primarily of Lactobacillus kefiri P-IF, a specific strain of L. kefiri with unique growth characteristics. The aim of this study was to examine the apoptotic effect of PFT on human multidrug-resistant (MDR) myeloid leukemia (HL60/AR) cells in vitro and explore the mechanistic approach underlying its effect. HL60/AR cells were cultured with PFT (0.6-5.0 mg/ml) for 3 days. The apoptotic effect of PFT was assessed through examination of percent apoptosis, caspase 3 activation, Bcl-2 expression levels and changes in mitochondrial membrane potential (MMP). PFT induced apoptosis in HL60/AR cells in a dose-dependent manner which was maximal at 67.5% for 5 mg/ml. Induction of apoptosis was associated with activation of caspase 3, decreased expression of Bcl-2 and decreased polarization of MMP. In addition, PFT showed a unique characteristic of piercing holes in HL60/AR cells, as indicated by AFM studies. This hole induction may be responsible for the apoptotic effect on cancer cells. These results suggest that PFT may act as a potential therapy for the treatment of MDR leukemia.
我们研究了一种新型益生菌发酵技术（PFT）酸奶粉产品的凋亡效应；PFT 是一种天然混合物，主要由 Lactobacillus kefiri P-IF 组成，这是一种具有独特生长特性的特定 L. kefiri 菌株。本研究旨在研究 PFT 对人多药耐药（MDR）髓系白血病（HL60/AR）细胞的体外凋亡作用，并探讨其作用的机制方法。HL60/AR 细胞用 PFT（0.6-5.0mg/ml）培养 3 天。通过检测凋亡细胞百分比、半胱氨酸蛋白酶 3 激活、Bcl-2 表达水平和线粒体膜电位（MMP）变化来评估 PFT 的凋亡作用。PFT 以剂量依赖性方式诱导 HL60/AR 细胞凋亡，5mg/ml 时最大可达 67.5%。诱导凋亡与半胱氨酸蛋白酶 3 激活、Bcl-2 表达降低和 MMP 极化降低有关。此外，AFM 研究表明，PFT 对 HL60/AR 细胞具有穿孔的独特特征。这种孔诱导可能是对癌细胞产生凋亡作用的原因。这些结果表明，PFT 可能作为治疗多药耐药白血病的潜在疗法。
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