Department of Genetics, Academy of Agriculture and the Biology Chair, Institute for Foreign Students, Sofia, Bulgaria.
Theor Appl Genet. 1972 Jan;42(7):306-9. doi: 10.1007/BF00277723.
The present report is based on tests using antigenic analysis of inbred lines and their hybrids for the prognosis of heterosis (hybrid vigour) in Zea mays.Inbred lines, whose hybrid vigour has been proved under field conditions, and their hybrids were characterized by the method of Grabar and Williams.The results of the electrophoretic and immunoelectrophoretic investigations show that the extracts from maize seeds of the inbred lines C-103, N-6 and WF-9 contain four protein fractions, while those of the hybrids N-6 x C-103 and WF-9 x N-6 also contain a fifth fraction.The immunoelectrophoretic pattern of the extracts from the hybrid seeds N-6 x C-103 shows that, with their homologous serum, they give a precipitation line (arc X) which is not obtained by the interaction of the parent line extracts with homologous and heterologous serums.In this case we probably have a protein synthesis in the hybrid which is caused by the deblocking of some links in the biosynthetic apparatus of the inbred lines.It was established that the extracts from the hybrid N-6 x C-103 gave a larger number of precipitation arcs with the heterologous serum anti C-103.The characterization of the antigenic structure of the inbred lines enables one to determine not only the fractions common to them but also the number of those fractions by which they differ from one another. Proceeding in this manner, one could accomplish an immunoelectrophoretic prognosis of heterosis and penetrate deeper into its essence.
本报告是基于对自交系及其杂种进行抗原分析的测试，以预测玉米杂种优势（杂种活力）的结果。在田间条件下已证明杂种活力的自交系及其杂种，采用格拉巴尔和威廉姆斯的方法进行了特征描述。电泳和免疫电泳研究的结果表明，自交系 C-103、N-6 和 WF-9 的玉米种子提取物含有四个蛋白质分数，而杂种 N-6 x C-103 和 WF-9 x N-6 的提取物也含有第五个分数。杂种种子 N-6 x C-103 的提取物的免疫电泳图谱表明，与同源血清反应时，它们会产生一条沉淀线（弧 X），而这是通过父本系提取物与同源和异源血清相互作用无法获得的。在这种情况下，我们可能在杂种中发生了蛋白质合成，这是由于自交系生物合成装置中的某些环节被解除了封锁。已经确定，杂种 N-6 x C-103 的提取物与异源血清抗 C-103 反应产生了更多的沉淀弧。自交系抗原结构的特征化不仅可以确定它们共有的分数，还可以确定它们彼此之间不同的分数数量。通过这种方式，可以实现杂种优势的免疫电泳预测，并更深入地了解其本质。
