Ballantine Lucy E, Ong Joanne, Midgley Angela, Watson Louise, Flanagan Brian F, Beresford Michael W
Department of Women's and Children's Health, Institute of Translational Medicine, University of Liverpool, Alder Hey Children's NHS Foundation Trust Hospital Eaton Road, Liverpool L12 2AP, UK.
Pediatr Rheumatol Online J. 2014 Jan 16;12:4. doi: 10.1186/1546-0096-12-4.
T cells are important to systemic lupus erythematosus (SLE) disease progression. This study determined the pro-inflammatory potential of T cells within the rare condition juvenile-onset SLE (JSLE).
IL-17A and Th1/Th2-related cytokine concentrations were measured in plasma/serum from JSLE patients (n = 19, n = 11) and HC (n = 18, n = 7). IL17A, RORC, IL23 and IL23R mRNA were measured in peripheral blood mononuclear cells (PBMCs) from JSLE and healthy controls (HC) (n = 12). Th17-associated cytokine expression was analysed in the supernatant of CD3/CD28 activated JSLE (n = 7) and HC (n = 6) PBMCs.
JSLE plasma IL-17A level (21.5 ± 5.2 pg/ml) was higher compared to HC (7.2 ± 2.5 pg/ml, p = 0.028). No differences were found in Th1/Th2 cytokines levels. IL = 17A (p = 0.022), IL-6 (p = 0.028) and IL-21 (p = 0.003) concentrations were increased in supernatants from activated JSLE PBMCs. IL-17 F (p = 0.50) and IL-22 (p = 0.43) were also increased but were not statistically significant. IL17A and IL23 mRNA was significantly higher in JSLE PBMCs (p = 0.018 and p = 0.01).
JSLE T cells have an increased ability to secrete Th17 associated cytokines once activated, which could contribute to the pro-inflammatory disease phenotype seen in these patients.
T细胞对系统性红斑狼疮（SLE）疾病进展至关重要。本研究确定了罕见的青少年型SLE（JSLE）中T细胞的促炎潜力。
测量了JSLE患者（n = 19，n = 11）和健康对照（HC）（n = 18，n = 7）血浆/血清中的IL-17A和Th1/Th2相关细胞因子浓度。测量了JSLE患者和健康对照（HC）（n = 12）外周血单个核细胞（PBMC）中的IL17A、RORC、IL23和IL23R mRNA。分析了CD3/CD28激活的JSLE（n = 7）和HC（n = 6）PBMC上清液中Th17相关细胞因子的表达。
与HC（7.2±2.5 pg/ml，p = 0.028）相比，JSLE血浆IL-17A水平（21.5±5.2 pg/ml）更高。Th1/Th2细胞因子水平未发现差异。激活的JSLE PBMC上清液中IL = 17A（p = 0.022）、IL-6（p = 0.028）和IL-21（p = 0.003）浓度升高。IL-17 F（p = 0.50）和IL-22（p = 0.43）也升高，但无统计学意义。JSLE PBMC中IL17A和IL23 mRNA显著更高（p = 0.018和p = 0.01）。
JSLE T细胞一旦被激活，分泌Th17相关细胞因子的能力增强，这可能导致这些患者出现促炎疾病表型。
