Quintana I Z, Silveira A V, Möller G
Department of Immunology, Stockholm University, Sweden.
Eur J Immunol. 1987 Sep;17(9):1343-9. doi: 10.1002/eji.1830170919.
Monoclonal antibodies directed against sheep erythrocytes of the isotypes IgG1, IgG2b and IgG2a were used to analyze the specificity of antibody-induced suppression of the immune response. It was first shown that all monoclonals reacted against different antigenic determinants and they all suppressed the immune response to sheep erythrocytes when given shortly after the antigen to more than 50% as compared to 90-96% inhibition obtained with a polyclonal antiserum. Increasing the doses of monoclonals did not increase suppression. However, two different monoclonals administered together caused an additive, but not a synergistic inhibitory effect. No enhancement of the immune response was observed with any of the Ig classes tested. These findings show that four different antigenic determinants on sheep erythrocytes induced the synthesis of corresponding antibodies, with little or no signs of a dominant determinant. Passively administered monoclonal antibodies, even at supraoptimal doses, never suppressed the immune response to the same extent as a polyclonal antiserum, suggesting that each monoclonal only suppressed the synthesis of the corresponding antibody and did not affect antibody synthesis to other determinants.
针对IgG1、IgG2b和IgG2a同种型羊红细胞的单克隆抗体被用于分析抗体诱导的免疫反应抑制的特异性。首先表明，所有单克隆抗体都针对不同的抗原决定簇起反应，并且当在抗原后不久给予时，它们都能抑制对羊红细胞的免疫反应，与多克隆抗血清获得的90 - 96%的抑制相比，抑制率超过50%。增加单克隆抗体的剂量并不会增加抑制作用。然而，一起给予两种不同的单克隆抗体产生了相加但非协同的抑制作用。在所测试的任何Ig类别中均未观察到免疫反应增强。这些发现表明，羊红细胞上的四种不同抗原决定簇诱导了相应抗体的合成，几乎没有或没有显性决定簇的迹象。被动给予的单克隆抗体，即使剂量超最佳，也从未像多克隆抗血清那样在相同程度上抑制免疫反应，这表明每种单克隆抗体仅抑制相应抗体的合成，而不影响对其他决定簇的抗体合成。
