Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia.
Department of Biochemistry and Molecular Biology, University of Córdoba, Severo Ochoa Building, Rabanales Campus, Highway A4 Km 396a, 14071-Córdoba, Spain; Maimonides Institute for Research in Biomedicine of Córdoba, Reina Sofía University Hospital, University of Córdoba, 14071-Córdoba, Spain.
Ecotoxicol Environ Saf. 2014 Feb;100:15-26. doi: 10.1016/j.ecoenv.2013.11.025. Epub 2013 Dec 20.
This study uses proteomics approach to assess the toxic effects of contaminants in the Mediterranean crab (Carcinus maenas) after transplantation into Téboulba fishing harbour. High levels of aliphatic and aromatic hydrocarbons were detected in sediments. Although their effects on vertebrates are well described, little is known about their early biological effects in marine invertebrates under realistic conditions. Protein expression profiles of crabs caged for 15, 30 and 60 days were compared to unexposed animals. Nineteen proteins with significant expression differences were identified by capLC-µESI-IT MS/MS and homology search on databases. Differentially expressed proteins were assigned to five different categories of biological function including: (1) chitin catabolism, (2) proteolysis, (3) exoskeleton biosynthesis, (4) protein folding and stress response, and (5) transport. The proteins showing major expression changes in C. maenas after different caging times may be considered as novel molecular biomarkers for effectively biomonitoring aquatic environment contamination.
本研究采用蛋白质组学方法评估地中海蟹（Carcinus maenas）移植到 Teboulba 渔港后污染物的毒性效应。沉积物中检测到高水平的脂肪烃和芳香烃。尽管它们对脊椎动物的影响已有很好的描述，但在现实条件下，对海洋无脊椎动物的早期生物学效应知之甚少。将在网笼中饲养 15、30 和 60 天的螃蟹与未暴露的动物进行比较。通过 capLC-µESI-IT MS/MS 和数据库同源性搜索，鉴定了 19 种具有显著表达差异的蛋白质。差异表达的蛋白质被分配到五个不同的生物学功能类别，包括：（1）甲壳质分解代谢，（2）蛋白水解，（3）外骨骼生物合成，（4）蛋白质折叠和应激反应，以及（5）运输。在不同的网笼时间后，C. maenas 中表达变化较大的蛋白质可被视为有效监测水生环境污染的新型分子生物标志物。
