Sozzani Rosangela, Busch Wolfgang, Spalding Edgar P, Benfey Philip N
Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, 1030 Vienna, Austria.
Trends Plant Sci. 2014 May;19(5):304-10. doi: 10.1016/j.tplants.2013.12.003. Epub 2014 Jan 13.
A variety of imaging methodologies are being used to collect data for quantitative studies of plant growth and development from living plants. Multi-level data, from macroscopic to molecular, and from weeks to seconds, can be acquired. Furthermore, advances in parallelized and automated image acquisition enable the throughput to capture images from large populations of plants under specific growth conditions. Image-processing capabilities allow for 3D or 4D reconstruction of image data and automated quantification of biological features. These advances facilitate the integration of imaging data with genome-wide molecular data to enable systems-level modeling.
目前正在使用多种成像方法从活体植物中收集数据,以进行植物生长发育的定量研究。可以获取从宏观到分子、从数周到数秒的多层次数据。此外,并行化和自动化图像采集技术的进步使通量能够在特定生长条件下从大量植物群体中捕获图像。图像处理能力允许对图像数据进行三维或四维重建,并自动量化生物学特征。这些进展促进了成像数据与全基因组分子数据的整合,以实现系统水平的建模。