Institut für Botanik, Mittlerer Dallenbergweg 64, D-8700, Würzburg, FRG.
Photosynth Res. 1986 Jan;10(1-2):51-62. doi: 10.1007/BF00024185.
A newly developed fluorescence measuring system is employed for the recording of chlorophyll fluorescence induction kinetics (Kautsky-effect) and for the continuous determination of the photochemical and non-photochemical components of fluorescence quenching. The measuring system, which is based on a pulse modulation principle, selectively monitors the fluorescence yield of a weak measuring beam and is not affected even by extremely high intensities of actinic light. By repetitive application of short light pulses of saturating intensity, the fluorescence yield at complete suppression of photochemical quenching is repetitively recorded, allowing the determination of continuous plots of photochemical quenching and non-photochemical quenching. Such plots are compared with the time courses of variable fluorescence at different intensities of actinic illumination. The differences between the observed kinetics are discussed. It is shown that the modulation fluorometer, in combination with the application of saturating light pulses, provides essential information beyond that obtained with conventional chlorophyll fluorometers.
一种新开发的荧光测量系统被用于记录叶绿素荧光诱导动力学（Kautsky 效应），并用于连续测定荧光猝灭的光化学和非光化学分量。该测量系统基于脉冲调制原理，选择性地监测弱测量光束的荧光产率，即使在极强的光激活光强度下也不受影响。通过重复施加短的饱和强度的光脉冲，重复记录完全抑制光化学猝灭时的荧光产率，从而允许测定光化学猝灭和非光化学猝灭的连续曲线。将这些曲线与不同光激活照度下的可变荧光的时间进程进行比较。讨论了观察到的动力学之间的差异。结果表明，调制荧光计与饱和光脉冲的应用相结合，提供了比传统叶绿素荧光计获得的信息更为重要的信息。
