Department of General Botany, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8006, Zürich, Switzerland.
Planta. 1974 Dec;118(4):323-32. doi: 10.1007/BF00385582.
A method for producing stratified and viable subcells, consisting of segments of the stalk, has been worked out in Acetabularia mediterranea (LmX). Centrifugation at low speed results in the formation of a cytoplasmic moiety at the centrifugal pole which contains ca. 90% of the aldolase activity that is localized in the chloroplasts and in the cytosol. The vacuolar moiety, which occupies 80-92% of the cell volume, stains with neutral red, i.e. is viable. It contains ca. 76% of the total RNase and 72% of the total phosphotase activity. It is concluded that a large proportion of these hydrolase activities is present in the vacuole.
已在中肋骨条藻（LmX）中制定了一种产生分层且有活力的亚细胞的方法，该方法由茎段组成。低速离心会在离心极处形成细胞质部分，其中约含有 90%的定位在叶绿体和细胞质溶胶中的醛缩酶活性。占据细胞体积 80-92%的液泡部分用中性红染色，即具有活力。它包含约 76%的总 RNase 和 72%的总磷酸酶活性。可以得出结论，这些水解酶活性的很大一部分存在于液泡中。
