RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Food Chem. 2014;152:251-60. doi: 10.1016/j.foodchem.2013.11.126. Epub 2013 Dec 1.
The aim of this work was to develop a simple and rapid in vitro evaluation method for screening and discovery of uncharacterised and untapped prebiotic foods. Using a NMR-based metabolomic approach coupled with multivariate statistical analysis, the metabolic profiles generated by intestinal microbiota after in vitro incubation with feces were examined. The viscous substances of Japanese bunching onion (JBOVS) were identified as one of the candidate prebiotic foods by this in vitro screening method. The JBOVS were primarily composed of sugar components, especially fructose-based carbohydrates. Our results suggested that ingestion of JBOVS contributed to lactate and acetate production by the intestinal microbiota, and were accompanied by an increase in the Lactobacillus murinus and Bacteroidetes sp. populations in the intestine and fluctuation of the host-microbial co-metabolic process. Therefore, our approach should be useful as a rapid and simple screening tool for potential prebiotic foods.
本工作旨在开发一种简单、快速的体外评价方法，用于筛选和发现未被描述和利用的益生元食品。采用基于 NMR 的代谢组学方法结合多变量统计分析，检测了肠道微生物群在体外与粪便孵育后产生的代谢谱。通过这种体外筛选方法，发现日本大葱粘性物质（JBOVS）是候选益生元食品之一。JBOVS 主要由糖组成，特别是基于果糖的碳水化合物。我们的结果表明，摄入 JBOVS 有助于肠道微生物群产生乳酸盐和乙酸盐，并伴随着肠道中乳酸乳杆菌和拟杆菌属的增加以及宿主-微生物共代谢过程的波动。因此，我们的方法可用作潜在益生元食品的快速、简单筛选工具。
