College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
College of Bioengineering, Beijing Polytechnic, No.1 Shaoyaoju, Beijing 100029, China.
Food Chem. 2014;152:386-90. doi: 10.1016/j.foodchem.2013.11.080. Epub 2013 Nov 22.
Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and (1)H and (13)C nuclear magnetic resonance (NMR) spectra.
蓝色金银花果实富含花色苷，具有许多有益的作用，如降低心血管疾病、糖尿病和癌症的风险。本文首次报道了采用正丁醇/叔丁基甲基醚/乙腈/水/三氟乙酸（2:2:1:5:0.01，v/v）两相溶剂体系的高速逆流色谱（HSCCC）在制备规模上从蓝色金银花果实粗提物中分离花色苷。每次进样 100mg 粗提物，可得到 22.8mg 矢车菊素 3-葡萄糖苷（C3G），纯度为 98.1%。通过电喷雾电离质谱（ESI/MS）和（1）H 和（13）C 核磁共振（NMR）谱对化合物进行了鉴定。
