Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
College of Chinese Material Medicine, Jilin Agricultural University, Changchun 130118, China.
Molecules. 2017 Nov 17;22(11):2002. doi: 10.3390/molecules22112002.
Secoiridoid and iridoid glycosides are the main active components of . In this work, one iridoid and three secoiridoid glycosides from have been purified by high-speed counter-current chromatography in two runs using different solvent systems. Ethyl acetate--butanol-water (2:1:3, //) was the optimum solvent system to purify ca. 4.36 mg of loganic acid, 3.05 mg of swertiamarin, and 35.66 mg of gentiopicroside with 98.1%, 97.2% and 98.6% purities, respectively, while 31.15 mg of trifloroside with 98.9% purity was separated using hexane-ethyl acetate-methanol-water (1:3:1:3, ///). The structures of the glycosides were identified by mass spectrometry and NMR. After separation, the anti-nitric oxide production effects of the compounds on lipopolysaccharide-induced BV-2 murine microglial cells were also evaluated. All of the compounds inhibited the production of nitric oxide in lipopolysaccharide-induced BV-2 cells with high cell viabilities in a concentration-dependent manner, which demonstrated that were able to be used as a nitric oxide inhibitor.
环烯醚萜苷和裂环环烯醚萜苷是 的主要活性成分。在这项工作中，使用两种不同的溶剂系统，通过高速逆流色谱法从 中分离得到一种裂环环烯醚萜苷和三种环烯醚萜苷。乙酸乙酯-正丁醇-水（2:1:3，//）是最佳溶剂系统，可分别纯化约 4.36mg 的(loganic acid)、3.05mg 的(swertiamarin)和 35.66mg 的(gentiopicroside)，纯度分别为 98.1%、97.2%和 98.6%，而用正己烷-乙酸乙酯-甲醇-水（1:3:1:3，///）可分离出 31.15mg 的(trifloroside)，纯度为 98.9%。通过质谱和 NMR 鉴定了糖苷的结构。分离后，还评估了化合物对脂多糖诱导的 BV-2 小胶质细胞产生一氧化氮的抑制作用。所有化合物均能抑制脂多糖诱导的 BV-2 细胞中一氧化氮的产生，且具有高细胞活力的浓度依赖性，这表明它们可作为一氧化氮抑制剂使用。
