Cao Kun, Qian Wei, Xu Yi, Zhou Zhen
Pharmaceutical and Biological Engineering College, Chongqing University of Technology, Chongqing 400054, P.R. China.
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, P.R. China.
Iran J Pharm Res. 2019 Summer;18(3):1499-1507. doi: 10.22037/ijpr.2019.1100755.
Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated from roots, which had exhibited a wide range of biological activities such as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed counter-current chromatography (HSCCC) was applied for the rapid preparative isolation of sesquiterpenes lactones from roots. A solvent optimization method for HSCCC was presented, i.e. the separation factors of compounds after the partition coefficient (K) of solvent system should be investigated. Using this method, 150 mg of costunolide, 140 mg of dehydrocostus and 15 mg of 10-methoxy-artemisinic acid with purities of 95%, 98%, and 98% were obtained within 150 min. The structures of three compounds were identified by mass spectrum (MS) and nuclear magnetic resonance (NMR) spectroscopy. These results offered an efficient strategy for separation of potentially health-relevant phytochemicals from roots.
倍半萜内酯，包括木香内酯和脱氢木香内酯，是从根部分离得到的，这些根部已展现出广泛的生物活性，如抗癌、抗炎、抗溃疡和免疫调节活性。高速逆流色谱法（HSCCC）被用于从根部快速制备分离倍半萜内酯。提出了一种HSCCC的溶剂优化方法，即应研究溶剂系统的分配系数（K）后化合物的分离因子。使用该方法，在150分钟内获得了纯度为95%的150毫克木香内酯、纯度为98%的140毫克脱氢木香内酯和纯度为98%的15毫克10-甲氧基青蒿酸。通过质谱（MS）和核磁共振（NMR）光谱鉴定了这三种化合物的结构。这些结果为从根部分离潜在的与健康相关的植物化学物质提供了一种有效的策略。
