College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Biosens Bioelectron. 2014 Jun 15;56:6-11. doi: 10.1016/j.bios.2013.12.064. Epub 2014 Jan 7.
Herein, a novel strategy for synthesizing fluorescent carbon nanoparticles (CPs) with a quantum yield of approximately 7.1% has been well established by mixing l-cysteine, diphosphorus pentoxide and water. Compared with other current protocols, the method described here displayed various advantages including friendly manipulations, low cost, and rapid reactions. Subsequently, we applied the CPs prepared here for detections of tetracyclines (TCs). Briefly, the fluorescence intensity of CPs was quenched once TCs were introduced. Based on this phenomenon, TCs were analyzed respectively accompanyed with satisfactory detection limits and linear ranges. Significantly, the practicability of this sensing method was further validated by assaying TC in human urine samples and pharmaceutical preparations, confirming its potential to broaden avenues for detecting TCs. Additionally, the CPs could serve as fluorescent powder and ink followed by a simple post-treatment, suggesting their promising applications.
在此，我们提出了一种新颖的策略，通过混合半胱氨酸、五氧化二磷和水来合成荧光碳纳米粒子（CPs），其量子产率约为 7.1%。与其他当前的方案相比，这里描述的方法具有多种优势，包括操作简便、成本低、反应迅速。随后，我们将在这里制备的 CPs 用于检测四环素（TCs）。简而言之，一旦引入 TCs，CPs 的荧光强度就会被猝灭。基于这一现象，TCs 可以分别进行分析，具有令人满意的检测限和线性范围。值得注意的是，该传感方法的实用性通过测定人尿液样品和药物制剂中的 TC 进一步得到了验证，证实了其在检测 TCs 方面的应用潜力。此外，CPs 可以作为荧光粉和墨水，经过简单的后处理，这表明它们具有广阔的应用前景。
