Center of Bacteriology, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, CEP 01246-902, São Paulo, SP, Brazil.
Center of Bacteriology, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, CEP 01246-902, São Paulo, SP, Brazil.
Diagn Microbiol Infect Dis. 2014 Apr;78(4):343-6. doi: 10.1016/j.diagmicrobio.2013.12.009. Epub 2013 Dec 21.
The aim of this study was to analyse the nested polymerase chain reaction (nested PCR) in human serum samples of patients with clinical manifestations of leptospirosis. The cases of leptospirosis were defined by the microagglutination test (MAT). The samples were collected in 2010. Of 1042 serum samples collected from 521 patients, 28 (5.4%) were considered positive cases of leptospirosis, and 493 (94.6%) were negative. Twenty-three confirmed cases had no MAT-detectable antibodies in the acute sample (mean of 5.6 days after onset). Nested PCR was positive in 22/23 (95.7%) patients during the acute phase of the disease, with negative results by MAT. Nested PCR was negative in all convalescent serum samples with positive results by MAT. All negative cases of leptospirosis were negative by nested PCR. The nested PCR is an alternative diagnostic tool for early detection of leptospires in sera during the first 7 days of the disease.
本研究旨在分析具有钩端螺旋体病临床表现的患者血清样本中的巢式聚合酶链反应（nested PCR）。钩端螺旋体病的病例通过微量凝集试验（MAT）定义。样本采集于 2010 年。从 521 例患者的 1042 份血清样本中，有 28 份（5.4%）被认为是钩端螺旋体病的阳性病例，493 份（94.6%）为阴性。23 例确诊病例在急性样本中（发病后 5.6 天的平均值）没有 MAT 检测到的抗体。在疾病的急性期，22/23（95.7%）患者的巢式 PCR 呈阳性，而 MAT 呈阴性。所有通过 MAT 呈阳性的恢复期血清样本的巢式 PCR 均为阴性。所有钩端螺旋体病的阴性病例的巢式 PCR 均为阴性。巢式 PCR 是一种替代诊断工具，可在疾病的最初 7 天内早期检测血清中的钩端螺旋体。
