Department of Aquiculture, College of Animal Science, South China Agricultural University, 483 Wushan Road, Guangzhou, 510642, People's Republic of China.
Parasitol Res. 2014 Mar;113(3):1239-45. doi: 10.1007/s00436-014-3763-6. Epub 2014 Jan 22.
Tegument antigens of tapeworm play an important role in modulation of host response and parasite survival. Characterizing appropriate antigens for parasite infection diagnosis and vaccination is rational and could have both economic and epidemiological significance in poultry industry. In the present study, a major protoscolex homologue (named RT10) of Echinococcus and Taenia spp. was amplified from Raillietina tetragona cestode. The RT10 cDNA was 1,877 bp long containing an open reading frame of 1,683 bp nucleotides, which encoded a deduced protein of 560 amino acids with an isoelectric point of 6.33. Secondary structure analysis demonstrated that RT10 was both hydrophilic and antigenic, and possessed N-terminal FERM domain and C-terminal ERM domain, respectively. With the same structural properties of previously reported antigens from Echinococcus and Taenia spp., RT10 tegument antigen had a more than 82% similarity in nucleotide level with initially reported antigens from Echinococcus and Taenia spp., and a more than 83% similarity in protein level, with the highest similarity of 85.2% to Taenia antigen H17g. In addition, phylogenetic analysis illustrated a high consistency between different genus antigens and evolutionary branching. Although the detailed function of RT10 is still unknown, the high sequence conservation and structural similarity to formerly identified tegument antigens from Echinococcus and Taenia spp. suggested that RT10 may play a similar role as the previous reported antigens between cestode and host. It is significant to clarify the antigenic and serodiagnostic characteristics in the subsequent work.
绦虫的表皮抗原在调节宿主反应和寄生虫存活方面发挥着重要作用。鉴定适合寄生虫感染诊断和疫苗接种的适当抗原是合理的，这在禽类养殖行业具有经济和流行病学意义。本研究从瑞利绦虫中扩增了一种棘球蚴和带绦虫属的原头节同源物（命名为 RT10）。RT10 cDNA 长 1877bp，包含一个 1683bp 的开放阅读框，编码一个 560 个氨基酸的推定蛋白，等电点为 6.33。二级结构分析表明，RT10 既亲水又具有抗原性，分别具有 N 端 FERM 结构域和 C 端 ERM 结构域。与先前报道的棘球蚴和带绦虫属的抗原具有相同的结构特性，RT10 表皮抗原在核苷酸水平上与最初报道的棘球蚴和带绦虫属的抗原具有 82%以上的相似性，在蛋白质水平上具有 83%以上的相似性，与 Taenia 抗原 H17g 的最高相似性为 85.2%。此外，系统发育分析表明不同属的抗原之间具有高度一致性和进化分支。尽管 RT10 的详细功能尚不清楚，但它与先前鉴定的棘球蚴和带绦虫属的表皮抗原在核苷酸和蛋白质水平上具有高度的序列保守性和结构相似性，表明 RT10 可能在绦虫和宿主之间发挥与先前报道的抗原相似的作用。在随后的工作中阐明其抗原性和血清学诊断特征具有重要意义。
