Benitez L, Harrison L J, Parkhouse R M, Gonzalez L M, Gottstein B, Garate T
Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, CNM, Madrid, Spain.
Parasitol Res. 1998 May;84(5):426-31. doi: 10.1007/s004360050423.
A clone (R-Tso18) was isolated from a Taenia saginata oncosphere cDNA library by screening with sera from rabbits immunised with oncosphere extract. It contained a full-length cDNA sequence of 1893 bp with an open reading frame of 1680 bp, corresponding to 559 amino acids with a deduced molecular mass of 65.173 kDa and an isoelectric point of 6.08. The R-Tso18 protein showed 80-84% nucleotide identity with the major protoscolex surface antigens of Echinococcus multilocularis (EM10) and E. granulosus (EG10). Preliminary immunogenicity studies employing the radiolabeled R-Tso18 protein in immune co-precipitation assays indicated sero-positivity for T. saginata-infected calf sera (6/13), T. solium cysticercosis human (7/22) and pig (2/2) sera and E. multilocularis (6/10)- and E. granulosus (1/12)-infected human sera, whereas other helminth-infection sera were negative. As immuno-precipitation is a relatively insensitive assay, it was concluded that further studies on the diagnostic potential of the purified recombinant R-Tso18 antigen, or its peptides, are merited.
通过用六钩蚴提取物免疫的兔血清筛选，从牛带绦虫六钩蚴cDNA文库中分离出一个克隆（R-Tso18）。它包含一个1893 bp的全长cDNA序列，开放阅读框为1680 bp，对应559个氨基酸，推导分子量为65.173 kDa，等电点为6.08。R-Tso18蛋白与多房棘球绦虫（EM10）和细粒棘球绦虫（EG10）的主要原头节表面抗原具有80-84%的核苷酸同一性。在免疫共沉淀试验中使用放射性标记的R-Tso18蛋白进行的初步免疫原性研究表明，牛带绦虫感染的小牛血清（6/13）、猪带绦虫囊尾蚴病患者（7/22）和猪（2/2）血清以及多房棘球绦虫（6/10）和细粒棘球绦虫（1/12）感染的人血清呈血清阳性，而其他蠕虫感染血清为阴性。由于免疫沉淀是一种相对不敏感的检测方法，因此得出结论，值得对纯化的重组R-Tso18抗原或其肽的诊断潜力进行进一步研究。
