Wang Jin, Xu Guoxing, Shen Feng, Kang Yifan
Department of Orthopedics, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China.
Tumour Biol. 2014 May;35(5):4859-65. doi: 10.1007/s13277-014-1637-2. Epub 2014 Jan 22.
In this study, we investigated the roles of miR-132 in tumor growth of osteosarcoma. We found that overexpression of miR-132 significantly suppressed in vitro cell proliferation and in vivo tumor growth. In addition, miR-132 overexpression induced G1/S cell cycle arrest of osteosarcoma cells. Further study showed that miR-132 could interact with the 3'-untranslated region of cyclin E1 (CCNE1) gene and repress its expression. Re-expression of CCNE1 (without the 3'UTR) could partially abrogate the miR-132-induced cell proliferation inhibition. Of significance, contrary to CCNE1, expression level of miR-132 was significantly lower in osteosarcoma tissues than in the adjacent normal tissues. Taken together, these results indicate that miR-132 functions as a tumor suppressor in osteosarcoma and that its suppressive effects are mediated chiefly by repressing CCNE1 expression.
在本研究中，我们调查了miR-132在骨肉瘤肿瘤生长中的作用。我们发现，miR-132的过表达显著抑制体外细胞增殖和体内肿瘤生长。此外，miR-132过表达诱导骨肉瘤细胞的G1/S期细胞周期阻滞。进一步研究表明，miR-132可与细胞周期蛋白E1（CCNE1）基因的3'非翻译区相互作用并抑制其表达。CCNE1（无3'UTR）的重新表达可部分消除miR-132诱导的细胞增殖抑制。重要的是，与CCNE1相反，miR-132在骨肉瘤组织中的表达水平显著低于相邻正常组织。综上所述，这些结果表明miR-132在骨肉瘤中起肿瘤抑制作用，其抑制作用主要通过抑制CCNE1表达来介导。
