生物信息学筛选子宫内膜癌候选生物标志物及其预后价值。
Bioinformatic screening for candidate biomarkers and their prognostic values in endometrial cancer.
机构信息
Department of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical University, Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor, Ministry of Education, Nanning, Guangxi, 530021, People's Republic of China.
Department of Gynecology and obstetrics, Shangyu People's Hospital, Shangyu, Zhejiang, 312300, People's Republic of China.
出版信息
BMC Genet. 2020 Sep 22;21(1):113. doi: 10.1186/s12863-020-00898-4.
BACKGROUND
Endometrial cancer is a common gynecological cancer with annually increasing incidence worldwide. However, the biomarkers that provide prognosis and progression for this disease remain elusive.
RESULTS
Two eligible human endometrial cancer datasets (GSE17025 and GSE25405) were selected for the study. A total of 520 differentially expressed mRNAs and 30 differentially expressed miRNAs were identified. These mRNAs were mainly enriched in cell cycle, skeletal system development, vasculature development, oocyte maturation, and oocyte meiosis signalling pathways. A total of 160 pairs of differentially expressed miRNAs and mRNAs, including 22 differentially expressed miRNAs and 71 overlapping differentially expressed mRNAs, were validated in endometrial cancer samples using starBase v2.0 project. The prognosis analysis revealed that Cyclin E1 (CCNE1, one of the 82 hub genes, which correlated with hsa-miR-195 and hsa-miR-424) was significantly linked to a worse overall survival in endometrial cancer patients.
CONCLUSIONS
The hub genes and differentially expressed miRNAs identified in this study might be used as prognostic biomarkers for endometrial cancer and molecular targets for its treatment.
背景
子宫内膜癌是一种常见的妇科癌症，全球发病率呈逐年上升趋势。然而，提供该疾病预后和进展的生物标志物仍难以确定。
结果
本研究选取了 2 个人类子宫内膜癌数据集（GSE17025 和 GSE25405）。共鉴定出 520 个差异表达的 mRNAs 和 30 个差异表达的 miRNAs。这些 mRNAs 主要富集在细胞周期、骨骼系统发育、血管发育、卵母细胞成熟和卵母细胞减数分裂信号通路中。使用 starBase v2.0 项目在子宫内膜癌样本中验证了 160 对差异表达的 miRNAs 和 mRNAs，包括 22 个差异表达的 miRNAs 和 71 个重叠的差异表达的 mRNAs。预后分析表明，细胞周期蛋白 E1（CCNE1，与 hsa-miR-195 和 hsa-miR-424 相关的 82 个枢纽基因之一）与子宫内膜癌患者的总体生存率显著相关。
结论
本研究中鉴定的枢纽基因和差异表达的 miRNAs 可能可用作子宫内膜癌的预后生物标志物和治疗的分子靶点。
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