Masuzawa Toshiyuki, Masuda Shou, Fukui Takashi, Okamoto Yoshihiro, Bataa Jantsandoo, Oikawa Yosaburo, Ishiguro Fubito, Takada Nobuhiro
Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science.
Jpn J Infect Dis. 2014;67(1):47-9. doi: 10.7883/yoken.67.47.
A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).
在蒙古色楞格省开展了一项分子流行病学调查，以鉴定蜱传疾病病原体嗜吞噬细胞无形体和广义伯氏疏螺旋体。该调查是针对一名疑似感染嗜吞噬细胞无形体的患者进行的。2012年，通过在植被上挥旗采样法共采集了129只饥饿的全沟硬蜱成虫。分别针对16S rDNA（rrs）和5S-23S基因间隔区，通过聚合酶链反应（PCR）检测嗜吞噬细胞无形体和伯氏疏螺旋体属。嗜吞噬细胞无形体和广义伯氏疏螺旋体的感染率分别为6.2%和55.0%。129只蜱中有6只（4.9%）同时感染了嗜吞噬细胞无形体和广义伯氏疏螺旋体。在伯氏疏螺旋体属中，流行率最高的是20047型伽氏疏螺旋体（26.3%），其次是阿氏疏螺旋体（7.8%）和NT29型伽氏疏螺旋体（7.0%）。此外，还检测到同时感染阿氏疏螺旋体和20047型伽氏疏螺旋体的蜱（7.8%）以及同时感染NT29型和20047型伽氏疏螺旋体的蜱（6.2%）。
