蒙古全沟硬蜱中嗜吞噬细胞无形体和伯氏疏螺旋体的PCR检测
PCR detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus ticks in Mongolia.
作者信息
Masuzawa Toshiyuki, Masuda Shou, Fukui Takashi, Okamoto Yoshihiro, Bataa Jantsandoo, Oikawa Yosaburo, Ishiguro Fubito, Takada Nobuhiro
机构信息
Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science.
出版信息
Jpn J Infect Dis. 2014;67(1):47-9. doi: 10.7883/yoken.67.47.
A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S-23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).
在蒙古色楞格省开展了一项分子流行病学调查，以鉴定蜱传疾病病原体嗜吞噬细胞无形体和广义伯氏疏螺旋体。该调查是针对一名疑似感染嗜吞噬细胞无形体的患者进行的。2012年，通过在植被上挥旗采样法共采集了129只饥饿的全沟硬蜱成虫。分别针对16S rDNA（rrs）和5S-23S基因间隔区，通过聚合酶链反应（PCR）检测嗜吞噬细胞无形体和伯氏疏螺旋体属。嗜吞噬细胞无形体和广义伯氏疏螺旋体的感染率分别为6.2%和55.0%。129只蜱中有6只（4.9%）同时感染了嗜吞噬细胞无形体和广义伯氏疏螺旋体。在伯氏疏螺旋体属中，流行率最高的是20047型伽氏疏螺旋体（26.3%），其次是阿氏疏螺旋体（7.8%）和NT29型伽氏疏螺旋体（7.0%）。此外，还检测到同时感染阿氏疏螺旋体和20047型伽氏疏螺旋体的蜱（7.8%）以及同时感染NT29型和20047型伽氏疏螺旋体的蜱（6.2%）。
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