Department of Microbiology and Immunology, University of Michigan Medical School, 5622 Medical Sciences Bldg, II, 1150 West Medical Center Drive, 48109-5620, Ann Arbor, USA.
Microbiome. 2013 Feb 18;1(1):7. doi: 10.1186/2049-2618-1-7.
Murine norovirus (MNV) is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease (IBD). Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice.
The composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL/6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection.
This is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL/6 mice.
鼠诺如病毒（MNV）是研究小鼠最常见的胃肠道病原体，可改变炎症性肠病（IBD）的生物医学小鼠模型的研究结果。尽管有迹象表明，改变的微生物群是 IBD 的一个危险因素，但 MNV 感染对鼠肠道微生物群的反应尚未被检测到。MNV 感染引起的微生物群破坏可能会引入研究实验中观察到的混杂效应。因此，本研究调查了 MNV 感染对野生型小鼠肠道微生物群的影响。
在 MNV 感染后，通过 16S rRNA 编码基因焦磷酸测序，对瑞士 Webster 近交系和 C57BL/6 近交系的肠道微生物群进行了时间分析。用持续和非持续的 MNV 株感染小鼠，并分析组织相关或粪便相关的微生物群。感染小鼠肠道细菌群落的分析在门和科水平上与未感染对照相比没有明显差异，无论是在组织相关样本还是粪便中，并且在感染后随着时间的推移也没有明显差异，这表明野生型小鼠的肠道微生物群对 MNV 感染后的破坏具有很强的抵抗力。
这是首次描述 MNV 感染后的肠道微生物群的研究，并表明急性或持续的 MNV 感染与瑞士 Webster 和 C57BL/6 小鼠微生物群落的主要破坏无关。
