First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu, 431-3192, Japan.
Dig Dis Sci. 2014 Jul;59(7):1415-27. doi: 10.1007/s10620-013-3023-0. Epub 2014 Jan 23.
Trinitrobenzenesulfonic acid (TNBS)-induced colitis is one of the most widely used experimental colitis models. However, there is no standard procedure for inducing colitis by TNBS because it is difficult to achieve a uniform distribution of colitis. We have developed a modified method of murine TNBS-induced colitis that involves inhalation anesthesia with sevoflurane combined with both single and repeated TNBS administrations.
To compare the usefulness of our newly developed method for inducing murine TNBS-induced colitis with that of conventional intraperitoneal anesthesia.
TNBS in ethanol was administered to C57BL/6J mice held in an inverted vertical position either under continuous inhalation anesthesia with sevoflurane, in accordance with our newly developed method, or by intraperitoneal injection with 2.5 % avertin, in accordance with the conventional procedure. Body weight change, cytokine profile, and histological findings were examined during the course of colitis.
The dispersion of anesthesia time, TNBS retention time, and nadir weight during the course of colitis was decreased using the newly developed method compared with the conventional procedure. Optimization of the modified TNBS-induced colitis, as evidenced by the predominant expression of Th1 and Th17 cytokines on day 7, was attained by the injection of 2.25 mg TNBS in 55 % ethanol. Regulation of the TNBS retention time using inhalation anesthesia with sevoflurane allowed strict control of the disease severity of TNBS-induced colitis. Using the modified method we were also able to develop a chronic TNBS-induced colitis model by repeated TNBS administration without excessive mortality of the mice.
Our modified method for murine TNBS-induced colitis using continuous inhalation anesthesia with sevoflurane provides a better experimental colitis model following both single and repeated TNBS administrations.
三硝基苯磺酸（TNBS）诱导的结肠炎是最广泛使用的实验性结肠炎模型之一。然而，由于很难实现结肠炎的均匀分布，因此没有诱导结肠炎的标准程序。我们开发了一种改良的小鼠 TNBS 诱导结肠炎方法，该方法涉及使用七氟醚进行吸入麻醉，并结合单次和重复 TNBS 给药。
比较我们新开发的诱导小鼠 TNBS 诱导结肠炎的方法与传统腹腔麻醉的有用性。
将乙醇中的 TNBS 给予处于倒置垂直位置的 C57BL/6J 小鼠，要么根据我们新开发的方法进行连续吸入麻醉，要么根据传统程序进行腹腔注射 2.5%的 avertin。在结肠炎过程中检查体重变化、细胞因子谱和组织学发现。
与传统程序相比，使用新开发的方法可减少麻醉时间、TNBS 保留时间和结肠炎过程中的最低体重分散。通过注射 2.25 毫克 TNBS 在 55%乙醇中，优化改良的 TNBS 诱导结肠炎，表现为第 7 天 Th1 和 Th17 细胞因子的主要表达，达到了优化。使用七氟醚进行吸入麻醉来调节 TNBS 保留时间可以严格控制 TNBS 诱导结肠炎的严重程度。使用改良方法，我们还能够通过重复 TNBS 给药来开发慢性 TNBS 诱导结肠炎模型，而不会导致小鼠死亡率过高。
我们使用连续吸入麻醉的改良小鼠 TNBS 诱导结肠炎方法，在单次和重复 TNBS 给药后提供了更好的实验性结肠炎模型。
