Gao Linbo, Zhu Xinxin, Li Zhihui, Li Lijuan, Wang Tao, Hu Huaizhong, Guo Wanli, Chen Peng, Zhu Jingqiang, Zhang Lin
Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.
Tumour Biol. 2014 Apr;35(4):3861-5. doi: 10.1007/s13277-013-1512-6. Epub 2014 Jan 23.
The aim of this study was to evaluate whether an insertion/deletion polymorphism (rs3783553) locating in the miR-122 target gene IL1A 3' untranslated region was related to the risk of papillary thyroid carcinoma (PTC). Genomic DNA was extracted from peripheral venous blood of 273 patients with PTC and 509 controls. The IL1A rs3783553 polymorphism was genotyped by using a polymerase chain reaction assay. No significant difference of the distribution of the IL1A rs3783553 polymorphism was observed between PTC patients and controls. However, patients carrying the IL1A rs3783553 ins/ins genotype and ins allele had significantly decreased risks for developing T3 and T4 when compared with patients carrying the IL1A rs3783553 del/del genotype and del allele (ins/ins vs. del/del: OR = 0.22, 95% confidence interval (CI), 0.09-0.54; ins vs. del: OR = 0.58, 95% CI, 0.41-0.83, respectively). These results suggest that the rs3783553 polymorphism may be used as a genetic marker to predict the size/extension of PTC.
本研究旨在评估位于miR - 122靶基因IL1A 3'非翻译区的插入/缺失多态性（rs3783553）是否与甲状腺乳头状癌（PTC）的发病风险相关。从273例PTC患者和509例对照的外周静脉血中提取基因组DNA。采用聚合酶链反应法对IL1A rs3783553多态性进行基因分型。PTC患者与对照之间未观察到IL1A rs3783553多态性分布的显著差异。然而，与携带IL1A rs3783553 del/del基因型和del等位基因的患者相比，携带IL1A rs3783553 ins/ins基因型和ins等位基因的患者发生T3和T4的风险显著降低（ins/ins与del/del相比：比值比（OR）= 0.22，95%置信区间（CI）为0.09 - 0.54；ins与del相比：OR = 0.58，95%CI为0.41 - 0.83）。这些结果表明，rs3783553多态性可作为预测PTC大小/范围的遗传标志物。
