巴西北部和东北部患者样本中IFNGR1基因（rs137854905）、GSTT1基因（rs71748309）和GSTP1基因（rs1695）的单核苷酸多态性与胃癌发生的关联研究
Association study of SNPs of genes IFNGR1 (rs137854905), GSTT1 (rs71748309), and GSTP1 (rs1695) in gastric cancer development in samples of patient in the northern and northeastern Brazil.
作者信息
de Araújo Rubistenia Miranda Soares, de Melo Cynthia Farias Vieira, Neto Fidelis Manes, da Silva Juarez Nóbrega, Soares Leonardo Ferreira, de Arruda Cardoso Smith Marilia, Sousa Edilson Carvalho, Burbano Rommel Mario Rodríguez, de Medeiros Arnaldo Correia, Lima Eleonidas Moura
机构信息
Graduate Program in Cellular and Molecular Biology and Laboratory of Structural Molecular Biology and Oncogenetics-LBMEO, Department of Molecular Biology, Federal University of Paraíba UFPB, 58051-900, Joao Pessoa, Paraiba, Brazil.
出版信息
Tumour Biol. 2014 May;35(5):4983-6. doi: 10.1007/s13277-014-1656-z. Epub 2014 Jan 23.
Cancer is a multifactorial disease with a high mortality rate in Brazil and worldwide. Gastric cancer (GC) is considered the fourth type of malignancy more frequent in the population worldwide and the second leading cause of death. This work aimed to evaluate single nucleotide polymorphisms (SNPs) of IFNGR1, GSTT1, and GSTP1 genes samples in gastric cancer. We analyzed 60 samples of gastric cancer, 26 diffuse and 34 intestinal types, totaling 120 alleles for each SNP. The results were obtained by PCR and allele-specific PCR. Statistical analyzes performed using BioEstat 5.0 software, applying the Fisher's exact test and chi-square. Only the SNP gene GSTP1 (rs1695) were significantly associated with gastric cancer in the samples analyzed (χ(2) = 8.73, P < 0.05). Our results suggest that the GSTP1 gene SNP (rs1695) can be considered a risk factor associated with gastric carcinogenesis.
癌症是一种多因素疾病，在巴西乃至全球都有很高的死亡率。胃癌（GC）被认为是全球人群中第四常见的恶性肿瘤类型，也是第二大死亡原因。这项工作旨在评估胃癌样本中IFNGR1、GSTT1和GSTP1基因的单核苷酸多态性（SNP）。我们分析了60份胃癌样本，其中26份为弥漫型，34份为肠型，每个SNP共有120个等位基因。结果通过聚合酶链反应（PCR）和等位基因特异性PCR获得。使用BioEstat 5.0软件进行统计分析，应用Fisher精确检验和卡方检验。在所分析的样本中，只有SNP基因GSTP1（rs1695）与胃癌显著相关（χ(2) = 8.73，P < 0.05）。我们的结果表明，GSTP1基因SNP（rs1695）可被视为与胃癌发生相关的危险因素。
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