DNA修复基因ERCC8标签单核苷酸多态性在胃癌中的检测及临床意义
Detection and clinical significance of DNA repair gene ERCC8 tag SNPs in gastric cancer.
作者信息
Lu Xingre, Chen Fengyu, Liu Xiaowen, Yuan Diao, Zi Yunju, He Xiang, Zhu Deyong
机构信息
Department of Clinical Laboratory, The People's Hospital of Wenshan Prefecture, Yunnan, China.
出版信息
Turk J Gastroenterol. 2018 Jul;29(4):392-396. doi: 10.5152/tjg.2018.17662.
BACKGROUND/AIMS: Excision repair cross-complementing group 8 (ERCC8) is one of the members of the nucleotide excision repair pathway. This study aimed to explore the association between ERCC8 tag single nucleotide polymorphisms (SNPs) and gastric cancer.
MATERIALS AND METHODS
Totally, 120 patients with gastric cancer treated from March 2010 to March 2011 were selected as the observation group and 120 healthy individuals were selected as the control group during the same period. The Sequenom MassARRAY system was used to identify genotypes in these samples. The genetic locus of ERCC8 tag SNPs and the relevance of gastric cancer risk to the different ERCC8 genotypes alone or in combination with Helicobacter pylori infection were observed and analyzed. The AA, GA, and GG genotypes on rs158572 and rs158916 in the observation and control groups were compared.
RESULTS
The results showed that the odds ratio of the different ERCC8 rs158572 and rs158916 genotypes was not significantly increased in the observation group compared with that in the control group. By contrast, in patients with H. pylori infection, the ERCC8 rs158572 GA/GG and rs158916 TT genotypes showed a 7.921-fold and 8.021-fold [95% confidence interval (CI)=4.022-15.921, p=0.029 and 95% CI=3.021-15.092, p=0.021, respectively] increased risk of gastric cancer than the AA and CT/CC genotypes, respectively.
CONCLUSION
Helicobacter pylori infection combined with ERCC8 rs158572 and rs158916 can be used as a predictive index of gastric cancer occurrence.
背景/目的：切除修复交叉互补基因8（ERCC8）是核苷酸切除修复途径的成员之一。本研究旨在探讨ERCC8标签单核苷酸多态性（SNP）与胃癌之间的关联。
材料与方法
选取2010年3月至2011年3月期间接受治疗的120例胃癌患者作为观察组，同期选取120例健康个体作为对照组。使用Sequenom MassARRAY系统对这些样本进行基因分型。观察并分析ERCC8标签SNP的基因位点以及不同ERCC8基因型单独或与幽门螺杆菌感染联合与胃癌风险的相关性。比较观察组和对照组中rs158572和rs158916位点的AA、GA和GG基因型。
结果
结果显示，与对照组相比，观察组中不同ERCC8 rs158572和rs158916基因型的优势比没有显著增加。相比之下，在幽门螺杆菌感染患者中，ERCC8 rs158572的GA/GG基因型和rs158916的TT基因型患胃癌的风险分别比AA和CT/CC基因型高7.921倍和8.021倍[95%置信区间（CI）=4.022 - 15.921，p = 0.029；95% CI = 3.021 - 15.092，p = 0.021]。
结论
幽门螺杆菌感染联合ERCC8 rs158572和rs158916可作为胃癌发生的预测指标。
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