药用党参属植物中聚乙炔类、苯丙素类和吡咯烷类生物碱的高效液相色谱/紫外分析

HPLC/UV analysis of polyacetylenes, phenylpropanoid and pyrrolidine alkaloids in medicinally used Codonopsis species.

作者信息

He Jing-Yu, Zhu Shu, Komatsu Katsuko

机构信息

Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.

出版信息

Phytochem Anal. 2014 May-Jun;25(3):213-9. doi: 10.1002/pca.2494. Epub 2014 Jan 23.

DOI:10.1002/pca.2494

PMID:24453136

Abstract

INTRODUCTION

Codonopsis Radix is commonly used as a tonic in traditional Chinese medicine. However, there is no suitable method to assess the quality of Codonopsis Radix based on multiple components having potential bioactivities.

OBJECTIVE

To establish a HPLC/UV method for simultaneous quantitation of polyacetylenes (lobetyol, lobetyolin, lobetyolinin, cordifolioidyne B), phenylpropanoid (tangshenoside I) and pyrrolidine alkaloids (codonopyrrolidiums A, B) in Codonopsis Radix.

METHODS

Large-scale methanol extraction of Codonopsis Radix, followed by chromatographic separation, provided the seven analytes for quantitation standards. Ultrasound-assisted methanol extracts of samples were analysed using reversed phase, gradient elution HPLC monitored at 215 nm.

RESULTS

The method developed allowed efficient separation of the seven compounds and the detection and quantitation limits of the seven analytes were 0.10-0.32 µg/mL and 0.35-1.07 µg/mL, respectively. All calibration curves showed good linearities (r>0.9993) within the test ranges. Intraday and interday precisions were good with RSD<2.84%. The recoveries of all analytes ranged from 95.8 to 104.7%.

CONCLUSION

HPLC/UV is an efficient and accurate method of analysis for simultaneous quantitation of seven components in Codonopsis Radix.

摘要

引言

党参在传统中药中常用作滋补品。然而，目前尚无基于多种具有潜在生物活性成分来评估党参质量的合适方法。

目的

建立一种高效液相色谱/紫外检测法（HPLC/UV），用于同时定量测定党参中的聚乙炔类化合物（洛贝醇、洛贝亭、洛贝亭宁、党参炔苷B）、苯丙素类化合物（党参苷I）和吡咯烷生物碱（党参吡咯烷A、B）。

方法

采用大规模甲醇提取党参，随后进行色谱分离，得到七种分析物作为定量标准品。采用反相梯度洗脱高效液相色谱法，在215nm波长下监测，对超声辅助甲醇提取物进行分析。

结果

所建立的方法能够有效分离这七种化合物，七种分析物的检测限和定量限分别为0.10 - 0.32μg/mL和0.35 - 1.07μg/mL。所有校准曲线在测试范围内均呈现良好线性（r > 0.9993）。日内和日间精密度良好，相对标准偏差（RSD）< 2.84%。所有分析物的回收率在95.8%至104.7%之间。