Wistar大鼠胫神经在有间隙和无间隙的一期神经缝合中，包裹静脉段后的再生研究。
Study of tibial nerve regeneration in Wistar rats in primary neurorrhaphy with and without gap, wrapped in vein segments.
作者信息
Bastos Dos Santos Ewerton, Fernandes Marcela, Gomes Dos Santos João Baptista, Mattioli Leite Vilnei, Valente Sandra Gomes, Faloppa Flávio
机构信息
Universidade Federal de São Paulo (UNIFESP) - São Paulo, SP, Brazil.
出版信息
Acta Ortop Bras. 2012;20(3):165-9. doi: 10.1590/S1413-78522012000300006.
OBJECTIVE
This study compared nerve regeneration in Wistar rats, using epineural neurorrhaphy with a gap of 1.0 mm and without a gap, both wrapped with jugular vein tubes. Motor neurons in the spinal cord between L3 and S1 were used for the count, marked by exposure of the tibial nerve to Fluoro-Gold (FG).
METHOD
The tibial nerves on both sides were cut and sutured, with a gap on one side and no gap in the other. The sutures were wrapped with a jugular vein. Four months after surgery the tibial nerves were exposed to Fluoro-Gold and the motor neuron count performed in the spinal cord.
RESULTS
The results were statistically analyzed by the paired Wilcoxon test. There was a statistical difference between the groups with and without gap in relation to the motor neuron count (p=0.013).
CONCLUSION
The epineural neurorraphy without gap wrapped with jugular vein showed better results for nerve regeneration than the same procedure with gap.
LEVEL OF EVIDENCE
Experimental Study .
目的
本研究比较了Wistar大鼠的神经再生情况，采用包裹颈静脉管的无间隙和有1.0mm间隙的神经外膜缝合术。通过将胫神经暴露于荧光金（FG）标记，对L3和S1之间脊髓中的运动神经元进行计数。
方法
双侧胫神经切断并缝合，一侧有间隙，另一侧无间隙。缝合处用颈静脉包裹。术后4个月，将胫神经暴露于荧光金，并对脊髓中的运动神经元进行计数。
结果
采用配对Wilcoxon检验对结果进行统计学分析。有间隙组和无间隙组在运动神经元计数方面存在统计学差异（p = 0.013）。
结论
包裹颈静脉的无间隙神经外膜缝合术在神经再生方面比有间隙的相同手术效果更好。
证据水平
实验研究。
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