Wei Bo, Zhang Rong-Zhi, Guo Juan-Juan, Liu Dan-Mei, Li Ai-Li, Fan Ren-Chun, Mao Long, Zhang Xiang-Qi
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, PR China.
National Key Facility for Crop Gene Resources and Genetic Improvement and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, PR China.
PLoS One. 2014 Jan 13;9(1):e84781. doi: 10.1371/journal.pone.0084781. eCollection 2014.
MADS-box genes are important transcription factors for plant development, especially floral organogenesis. Brachypodium distachyon is a model for biofuel plants and temperate grasses such as wheat and barley, but a comprehensive analysis of MADS-box family proteins in Brachypodium is still missing. We report here a genome-wide analysis of the MADS-box gene family in Brachypodium distachyon. We identified 57 MADS-box genes and classified them into 32 MIKC(c)-type, 7 MIKC*-type, 9 Mα, 7 Mβ and 2 Mγ MADS-box genes according to their phylogenetic relationships to the Arabidopsis and rice MADS-box genes. Detailed gene structure and motif distribution were then studied. Investigation of their chromosomal localizations revealed that Brachypodium MADS-box genes distributed evenly across five chromosomes. In addition, five pairs of type II MADS-box genes were found on synteny blocks derived from whole genome duplication blocks. We then performed a systematic expression analysis of Brachypodium MADS-box genes in various tissues, particular floral organs. Further detection under salt, drought, and low-temperature conditions showed that some MADS-box genes may also be involved in abiotic stress responses, including type I genes. Comparative studies of MADS-box genes among Brachypodium, rice and Arabidopsis showed that Brachypodium had fewer gene duplication events. Taken together, this work provides useful data for further functional studies of MADS-box genes in Brachypodium distachyon.
MADS盒基因是植物发育尤其是花器官发生过程中的重要转录因子。短柄草是生物燃料植物以及小麦和大麦等温带禾本科植物的模式植物，但对短柄草中MADS盒家族蛋白的全面分析仍缺失。我们在此报告对短柄草中MADS盒基因家族的全基因组分析。我们鉴定出57个MADS盒基因，并根据它们与拟南芥和水稻MADS盒基因的系统发育关系，将它们分为32个MIKC(c)型、7个MIKC*型、9个Mα型、7个Mβ型和2个Mγ型MADS盒基因。随后研究了详细的基因结构和基序分布。对它们染色体定位的研究表明，短柄草MADS盒基因均匀分布在五条染色体上。此外，在源自全基因组复制块的同线块上发现了五对II型MADS盒基因。然后我们对短柄草MADS盒基因在各种组织尤其是花器官中的表达进行了系统分析。在盐、干旱和低温条件下的进一步检测表明，一些MADS盒基因可能也参与非生物胁迫响应，包括I型基因。短柄草、水稻和拟南芥之间MADS盒基因的比较研究表明，短柄草的基因重复事件较少。综上所述，这项工作为进一步研究短柄草中MADS盒基因的功能提供了有用的数据。
