College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China.
Int J Biol Macromol. 2019 Aug 1;134:131-138. doi: 10.1016/j.ijbiomac.2019.04.198. Epub 2019 May 4.
Chitin-glucan complex (CGC), the main component of fungal cell wall, is reported to have wide applications in the food and pharmaceutical industries because of its physical and physiological activities. In this study, CGC was extracted from the fruiting body of Termitomyces albuminosus (Berk.) Heim with the treatments of deproteination, demineralization and depigmentation to obtain a yield of 13.46%, and its properties were investigated. The results indicated that CGC from T. albuminosus contained glucan and chitin in a molar ratio of 46:54, with very low contents of proteins and inorganic salts. The chitin in CGC was in the α-form, with crystallinity index of 64.81% and degree of acetylation of 65.40%. The surface morphology of CGC was dense and firm with no nanofibers and nanopores as observed by scanning electron microscopy, and the peak degradation temperature was determined to be 314.88 °C. This study suggested that CGC from T. albuminosus was promising to be an alternative source of crustacean chitinous products in the industry of food, medicine, waste water treatment and so on in the future.
几丁质-葡聚糖复合物（CGC）是真菌细胞壁的主要成分，由于其物理和生理活性，已被报道在食品和制药行业中有广泛的应用。在本研究中，从白肉齿菌（Termitomyces albuminosus（Berk.）Heim）的子实体中提取 CGC，经过脱蛋白、脱矿质和脱色处理，获得了 13.46%的产率，并对其性质进行了研究。结果表明，白肉齿菌来源的 CGC 中葡聚糖和几丁质的摩尔比为 46:54，其蛋白质和无机盐含量非常低。CGC 中的几丁质呈 α 型，结晶度指数为 64.81%，乙酰化度为 65.40%。扫描电子显微镜观察到 CGC 的表面形貌致密且坚固，没有纳米纤维和纳米孔，其峰值降解温度为 314.88°C。本研究表明，白肉齿菌来源的 CGC 有望成为甲壳质产品在食品、医药、废水处理等行业的替代来源。
