EED在多梳蛋白复合体调控中的核心作用。

Cao Qi, Wang Xiaoju, Zhao Meng, Yang Rendong, Malik Rohit, Qiao Yuanyuan, Poliakov Anton, Yocum Anastasia K, Li Yong, Chen Wei, Cao Xuhong, Jiang Xia, Dahiya Arun, Harris Clair, Feng Felix Y, Kalantry Sundeep, Qin Zhaohui S, Dhanasekaran Saravana M, Chinnaiyan Arul M

1] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA [2] Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA [3] Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, Texas 77030, USA [4] Cancer Center, Houston Methodist Research Institute, Houston, Texas 77030, USA.

Nat Commun. 2014;5:3127. doi: 10.1038/ncomms4127.

Polycomb repressive complexes 1 and 2 (PRC1 and 2) play a critical role in the epigenetic regulation of transcription during cellular differentiation, stem cell pluripotency and neoplastic progression. Here we show that the polycomb group protein EED, a core component of PRC2, physically interacts with and functions as part of PRC1. Components of PRC1 and PRC2 compete for EED binding. EED functions to recruit PRC1 to H3K27me3 loci and enhances PRC1-mediated H2A ubiquitin E3 ligase activity. Taken together, we suggest an integral role for EED as an epigenetic exchange factor coordinating the activities of PRC1 and 2.

多梳抑制复合物1和2（PRC1和PRC2）在细胞分化、干细胞多能性和肿瘤进展过程中的转录表观遗传调控中发挥关键作用。在此我们表明，多梳蛋白家族蛋白EED是PRC2的核心成分，它与PRC1发生物理相互作用并作为PRC1的一部分发挥功能。PRC1和PRC2的成分竞争与EED结合。EED的功能是将PRC1招募至H3K27me3位点，并增强PRC1介导的H2A泛素E3连接酶活性。综上所述，我们认为EED作为一种表观遗传交换因子，在协调PRC1和PRC2的活性方面发挥不可或缺的作用。