Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST) , 261 Cheom-dan Gwagi-ro, Buk-gu, Gwangju 500-712, Republic of Korea.
Department of Environmental Engineering, Seoul National University of Science and Technology, Seoul 139-743, Republic of Korea.
J Hazard Mater. 2014 Feb 28;267:206-13. doi: 10.1016/j.jhazmat.2013.12.061. Epub 2014 Jan 7.
The study of the bioconcentration of silver nanoparticles (AgNPs) is important to fully understand their hazard potential in the aquatic environment. We synthesized AgNPs radiolabeled with silver isotopes ((110m)Ag) to quantify the bioconcentration of AgNPs coated with citrate (AgNPs-CIT) and polyvinylpyrrolidone (AgNPs-PVP) in Japanese medaka, and to investigate the biodistribution of silver in organs, which were compared with (110m)AgNO3. BCF values were determined to be 39.8±7.4, 42.5±5.1 and 116.4±6.1Lkg(-1) for AgNPs-CIT, AgNPs-PVP and AgNO3, respectively. The release of more silver ions in AgNPs-PVP contributed to a different kinetic uptake pattern with AgNPs-CIT, which was similar to that of AgNO3. Bioconcentrated AgNPs in medaka were not observed to be eliminated, independent of surface coating differences, similarly to AgNO3. There was no difference in biodistribution in each organ before and after depuration in two types of AgNPs and AgNO3, all of which were mainly concentrated in the liver. This study quantified the bioconcentration and distribution of AgNPs and AgNO3 more precisely by utilizing a silver isotope, which is helpful in monitoring the toxicity of AgNPs to Japanese medaka.
研究银纳米粒子（AgNPs）的生物浓缩对于充分了解其在水生环境中的潜在危害非常重要。我们合成了放射性标记有银同位素（(110m)Ag）的 AgNPs，以定量研究柠檬酸（AgNPs-CIT）和聚乙烯吡咯烷酮（AgNPs-PVP）包裹的 AgNPs 在日本青鳉鱼中的生物浓缩，并研究了银在器官中的分布，将其与（110m)AgNO3 进行了比较。BCF 值分别为 39.8±7.4、42.5±5.1 和 116.4±6.1Lkg(-1)，用于 AgNPs-CIT、AgNPs-PVP 和 AgNO3。AgNPs-PVP 中释放出更多的银离子，导致其与 AgNPs-CIT 的吸收动力学模式不同，与 AgNO3 相似。与 AgNO3 相似，青鳉鱼中生物浓缩的 AgNPs 未被观察到消除，这与表面涂层的差异无关。在两种类型的 AgNPs 和 AgNO3 中，在净化前后，每个器官中的分布没有差异，所有这些都主要集中在肝脏中。本研究通过利用银同位素更精确地量化了 AgNPs 和 AgNO3 的生物浓缩和分布，这有助于监测 AgNPs 对日本青鳉鱼的毒性。
