Kareem Mohammed Abdul, Gadhamsetty Saayi Krushna, Shaik Althaf Hussain, Prasad E Maruthi, Kodidhela Lakshmi Devi
Department of Biochemistry, Indira Gandhi National Open University, New Delhi, India ; Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India.
Department of Microbiology-Immunology, Northwestern University, Chicago, USA ; Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India.
J Ayurveda Integr Med. 2013 Oct;4(4):216-23. doi: 10.4103/0975-9476.123704.
Nutmeg a well-known spice used as a folk medicine in India to treat stomach ailments. Worldwide it is commonly used for food preservation and fragrance. Abundant references were given for nutmeg in ayurveda, unani, and siddha as a single drug or as an important constituent in formulations.
In the present study, nutmeg aqueous extract (NMAET) was evaluated against isoproterenol (ISO)-induced hepatotoxicity and oxidative stress.
Antioxidant enzymes, liver functions tests, and lipid profile tests were performed using standard procedures. Histological examination of liver was done by fixing in formaldehyde solution and hematoxylin staining.
Oral administration of NMAET effectively inhibited the ISO-induced changes in the activities of hepatic marker and antioxidant enzymes in plasma and heart tissue along with lipid peroxidation levels. The liver sections of ISO administered rats showed massive fatty changes, necrosis, ballooning degeneration, and broad infiltration of the lymphocytes and the loss of cellular boundaries; these changes were completely absent in groups treated with extract. Analysis of variance and Duncan's Multiple Range tests were used to perform statistical analysis.
Results suggest that the NMAET possess significant potential as hepatoprotective and antioxidative agent against ISO-induced damage in rats.
肉豆蔻是一种著名的香料，在印度被用作治疗胃部疾病的民间药物。在全球范围内，它通常用于食品保存和增添香味。在阿育吠陀医学、尤纳尼医学和悉达医学中，肉豆蔻作为单一药物或配方中的重要成分有大量记载。
在本研究中，评估了肉豆蔻水提取物（NMAET）对异丙肾上腺素（ISO）诱导的肝毒性和氧化应激的作用。
采用标准程序进行抗氧化酶、肝功能测试和血脂谱测试。通过用甲醛溶液固定并进行苏木精染色对肝脏进行组织学检查。
口服NMAET有效抑制了ISO诱导的血浆和心脏组织中肝脏标志物和抗氧化酶活性的变化以及脂质过氧化水平。给予ISO的大鼠肝脏切片显示出大量脂肪变化、坏死、气球样变性、淋巴细胞广泛浸润以及细胞边界丧失；而提取物处理组则完全没有这些变化。采用方差分析和邓肯多重极差检验进行统计分析。
结果表明，NMAET作为一种抗ISO诱导的大鼠损伤的肝保护和抗氧化剂具有显著潜力。
