Kascsak R J, Rubenstein R, Merz P A, Tonna-DeMasi M, Fersko R, Carp R I, Wisniewski H M, Diringer H
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
J Virol. 1987 Dec;61(12):3688-93. doi: 10.1128/JVI.61.12.3688-3693.1987.
Antibody response in mice to scrapie-associated fibril proteins (protease-resistant proteins [PrPs]) was generated to different epitopes depending on the source of antigen. Mice responded differently to PrPs isolated from scrapie-infected animals of homologous (mouse) versus heterologous (hamster) species. An enzyme-linked immunosorbent assay established to monitor this antibody response in mice immunized with PrPs was unable to detect such a response in scrapie-infected mice. A monoclonal antibody (MAb), 263K 3F4, derived from a mouse immunized with hamster 263K PrPs reacted with hamster but not mouse PrPs. MAb 263K 3F4 also recognized normal host protein of 33 to 35 kilodaltons in brain tissue from hamsters and humans but not from bovine, mouse, rat, sheep, or rabbit brains. This is the first demonstration of epitope differences on this host protein in different species. The defining of various epitopes on PrP through the use of MAbs will lead to a better understanding of the relationship of PrPs to their host precursor protein and to the infectious scrapie agent.
小鼠对瘙痒病相关纤维蛋白（蛋白酶抗性蛋白[PrPs]）的抗体反应取决于抗原来源，针对不同表位产生反应。小鼠对从同源（小鼠）和异源（仓鼠）物种的瘙痒病感染动物中分离出的PrPs反应不同。建立的用于监测用PrPs免疫的小鼠中这种抗体反应的酶联免疫吸附测定法，无法在瘙痒病感染的小鼠中检测到这种反应。一种单克隆抗体（MAb），263K 3F4，由用仓鼠263K PrPs免疫的小鼠产生，它与仓鼠PrPs反应，但不与小鼠PrPs反应。MAb 263K 3F4还识别仓鼠和人类脑组织中33至35千道尔顿的正常宿主蛋白，但不识别牛、小鼠、大鼠、绵羊或兔脑组织中的该蛋白。这是首次证明不同物种中这种宿主蛋白上的表位差异。通过使用单克隆抗体确定PrP上的各种表位，将有助于更好地理解PrPs与其宿主前体蛋白以及传染性瘙痒病病原体之间的关系。
