Diringer H, Gelderblom H, Hilmert H, Ozel M, Edelbluth C, Kimberlin R H
Nature. 1983;306(5942):476-8. doi: 10.1038/306476a0.
The development of a short incubation model of scrapie (strain 263K), in golden hamsters has added impetus to the purification of the infectious agent. Our own attempts have been based on methods pioneered by Millson and developed by Prusiner. We present here results indicating that a purification factor of up to 10(4) with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70-300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz et al.. Material of molecular weight (Mr) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection.
在金黄仓鼠中建立羊瘙痒病（263K毒株）短潜伏期模型推动了感染因子纯化工作的开展。我们自己的尝试是基于米尔森开创并由普鲁辛纳改进的方法。我们在此展示的结果表明，现在有可能实现相对于蛋白质高达10⁴的纯化系数。来自大脑的高感染性组分沉降范围为70 - 300S，并且含有大量与默茨等人发现的羊瘙痒病相关纤维（SAF）极为相似的纤维。分子量为26,000的物质（可能是蛋白质）似乎是这些纤维的主要成分。感染性与纤维之间的关联引发了两种可能性：纤维是羊瘙痒病病原体的感染形式，或者它们是对羊瘙痒病感染的病理反应。
