Reich N, Pine R, Levy D, Darnell J E
Rockefeller University, New York, New York 10021-6399.
J Virol. 1988 Jan;62(1):114-9. doi: 10.1128/JVI.62.1.114-119.1988.
Interferon treatment of cell cultures results in the rapid transcriptional induction of a specific set of genes. In this paper we explore the effect of cellular infection by several adenoviruses, both wild type and mutant, on the expression of these genes. Infection with adenovirus induces the transcription of the interferon-stimulated genes in the absence of any protein synthesis. In fact, the inhibition of protein synthesis during a wild-type infection produces enhanced stimulation of transcription of these genes. Experiments with viral mutants indicate the ability to specifically suppress this transcription maps to the E1A gene. In addition, the E1A gene products are capable of suppressing the specific transcriptional induction of interferon-stimulated promoters during cotransfection experiments and therefore presumably during viral infection. The dual effect of adenovirus on the expression of interferon-stimulated genes may represent an example of action and evolutionary reaction between virus and host.
用干扰素处理细胞培养物会导致一组特定基因的快速转录诱导。在本文中，我们探究了几种野生型和突变型腺病毒对这些基因表达的细胞感染效应。在没有任何蛋白质合成的情况下，腺病毒感染会诱导干扰素刺激基因的转录。事实上，在野生型感染期间抑制蛋白质合成会增强对这些基因转录的刺激。用病毒突变体进行的实验表明，特异性抑制这种转录的能力定位于E1A基因。此外，在共转染实验期间，因此大概在病毒感染期间，E1A基因产物能够抑制干扰素刺激启动子的特异性转录诱导。腺病毒对干扰素刺激基因表达的双重作用可能代表了病毒与宿主之间作用和进化反应的一个例子。
