Montell C, Courtois G, Eng C, Berk A
Cell. 1984 Apr;36(4):951-61. doi: 10.1016/0092-8674(84)90045-x.
Rodent cells transformed by adenovirus 2 (Ad2) express two highly related viral proteins of 289 and 243 amino acids encoded in early region 1A (E1A). Transformation studies were performed with adenovirus mutants that express only one or the other E1A protein. We found that the 289 amino acid protein, which has transcription inducing activity, and the 243 amino acid protein, which has little if any of this activity, were both required to produce the fully transformed phenotype. Expression of either E1A protein induced a partially transformed phenotype. The 243 amino acid protein was particularly important for anchorage independent growth. As found in previous studies with several other E1A mutants, the process of transformation by the mutant that expresses the 243 amino acid protein only was cold-sensitive. While the 289 amino acid protein is the only E1A protein required for efficient viral replication under standard cell culture conditions, the 243 amino acid protein in addition to the 289 amino acid protein was found to be required for efficient viral replication in growth-arrested human cells.
被腺病毒2（Ad2）转化的啮齿动物细胞表达两种高度相关的病毒蛋白，它们由早期区域1A（E1A）编码，分别含289个和243个氨基酸。利用仅表达一种E1A蛋白的腺病毒突变体进行了转化研究。我们发现，具有转录诱导活性的289个氨基酸的蛋白和几乎没有这种活性的243个氨基酸的蛋白，都是产生完全转化表型所必需的。任一E1A蛋白的表达都会诱导部分转化表型。243个氨基酸的蛋白对于不依赖贴壁生长尤为重要。正如之前对其他几个E1A突变体的研究所发现的，仅表达243个氨基酸蛋白的突变体的转化过程对温度敏感。虽然在标准细胞培养条件下，289个氨基酸的蛋白是高效病毒复制所需的唯一E1A蛋白，但发现在生长停滞的人类细胞中，高效病毒复制除了需要289个氨基酸的蛋白外，还需要243个氨基酸的蛋白。
