香附子体外抗氧化及凋亡活性的评估

Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus.

作者信息

Soumaya Kilani-Jaziri, Zied Ghedira, Nouha Nasr, Mounira Krifa, Kamel Ghedira, Genviève Franca Dijoux Marie, Leila Ghedira Chekir

机构信息

Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.

Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie.

出版信息

Asian Pac J Trop Med. 2014 Feb;7(2):105-12. doi: 10.1016/S1995-7645(14)60004-3.

DOI:10.1016/S1995-7645(14)60004-3

PMID:24461522

Abstract

OBJECTIVE

To evaluate in vitro antioxidant and apoptotic activities of Cyperus rotundus (C. rotundus).

METHODS

The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both methanol and aqueous extracts (300, 150, and 50 μg/mL) were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system. However, 16, 8, and 4 mg/mL of each extract were tested to investigate their OH. formation scavenging potential. Aqueous extract (800, 400, and 200 μg/mL) and methanol extract (350, 175, and 88 μg/mL) were tested against lipid peroxidation, induced by 75 μM H2O2. The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated towards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by RMN spectroscopic analysis.

RESULTS

The methanol and aqueous extracts showed respectively, 88% and 19% inhibition of xanthine oxidase activity. Yet, the same extracts inhibited lipid peroxidation by 61.5% and 42.0%, respectively. Both extracts inhibited OH. formation by 27.1% and 25.3%, respectively. Only methanol extract induced DNA degradation. Orientin was determined as the major compound isolated from the butanol fraction of methanol extract.

CONCLUSIONS

It appears that C. rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.

摘要

目的

评估香附的体外抗氧化和凋亡活性。

方法

对香附地上部分的甲醇提取物和水提取物进行植物化学研究及抗氧化活性测定。此外，还研究了这些提取物的细胞毒性和凋亡活性。分离出甲醇提取物的主要化合物。通过黄嘌呤/黄嘌呤氧化酶测定系统评估甲醇提取物和水提取物（300、150和50μg/mL）的抗氧化活性。然而，测试每种提取物16、8和4mg/mL的浓度以研究其清除羟基自由基的潜力。测试水提取物（800、400和200μg/mL）和甲醇提取物（350、175和88μg/mL）对75μM过氧化氢诱导的脂质过氧化的抑制作用。通过MTT法评估两种提取物对K562和L1210细胞系的细胞毒性及细胞DNA片段化情况。从甲醇提取物的正丁醇部分获得主要化合物，并通过核磁共振光谱分析确定其结构。