Soumaya Kilani-Jaziri, Zied Ghedira, Nouha Nasr, Mounira Krifa, Kamel Ghedira, Genviève Franca Dijoux Marie, Leila Ghedira Chekir
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie; Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Universitéde Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12, Université de Monastir, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
Asian Pac J Trop Med. 2014 Feb;7(2):105-12. doi: 10.1016/S1995-7645(14)60004-3.
To evaluate in vitro antioxidant and apoptotic activities of Cyperus rotundus (C. rotundus).
The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both methanol and aqueous extracts (300, 150, and 50 μg/mL) were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system. However, 16, 8, and 4 mg/mL of each extract were tested to investigate their OH. formation scavenging potential. Aqueous extract (800, 400, and 200 μg/mL) and methanol extract (350, 175, and 88 μg/mL) were tested against lipid peroxidation, induced by 75 μM H2O2. The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated towards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by RMN spectroscopic analysis.
The methanol and aqueous extracts showed respectively, 88% and 19% inhibition of xanthine oxidase activity. Yet, the same extracts inhibited lipid peroxidation by 61.5% and 42.0%, respectively. Both extracts inhibited OH. formation by 27.1% and 25.3%, respectively. Only methanol extract induced DNA degradation. Orientin was determined as the major compound isolated from the butanol fraction of methanol extract.
It appears that C. rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.
评估香附的体外抗氧化和凋亡活性。
对香附地上部分的甲醇提取物和水提取物进行植物化学研究及抗氧化活性测定。此外，还研究了这些提取物的细胞毒性和凋亡活性。分离出甲醇提取物的主要化合物。通过黄嘌呤/黄嘌呤氧化酶测定系统评估甲醇提取物和水提取物（300、150和50μg/mL）的抗氧化活性。然而，测试每种提取物16、8和4mg/mL的浓度以研究其清除羟基自由基的潜力。测试水提取物（800、400和200μg/mL）和甲醇提取物（350、175和88μg/mL）对75μM过氧化氢诱导的脂质过氧化的抑制作用。通过MTT法评估两种提取物对K562和L1210细胞系的细胞毒性及细胞DNA片段化情况。从甲醇提取物的正丁醇部分获得主要化合物，并通过核磁共振光谱分析确定其结构。
甲醇提取物和水提取物分别对黄嘌呤氧化酶活性有88%和19%的抑制作用。然而，相同的提取物分别对脂质过氧化有61.5%和42.0%的抑制作用。两种提取物分别对羟基自由基的形成有27.1%和25.3%的抑制作用。只有甲醇提取物诱导DNA降解。确定从甲醇提取物的正丁醇部分分离出的松果菊苷为主要化合物。
香附提取物似乎有作为天然抗氧化剂和凋亡诱导剂的潜在用途。
