Kilani Soumaya, Ben Sghaier Mohamed, Limem Ilef, Bouhlel Ines, Boubaker Jihed, Bhouri Wissem, Skandrani Ines, Neffatti Aicha, Ben Ammar Ribai, Dijoux-Franca Marie Genviève, Ghedira Kamel, Chekir-Ghedira Leila
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculty of Pharmacy of Monastir, 5000 Monastir, Tunisia.
Bioresour Technol. 2008 Dec;99(18):9004-8. doi: 10.1016/j.biortech.2008.04.066. Epub 2008 Jun 5.
The in vitro antibacterial, antioxidant, cytotoxic and apoptotic activities from tubers extracts of Cyperus rotundus (Cyperaceae) were investigated. Antibacterial activity of different extracts was evaluated against five bacterial reference strains. A marked inhibitory effect was observed against Salmonella enteritidis, Staphylococcus aureus and Enterococcus faecalis with total oligomers flavonoids (TOFs) and ethyl acetate extracts. In addition to their antibacterial activity, the same extracts showed a significant ability to inhibit nitroblue tetrazolium reduction by the superoxide radical in a non-enzymatic superoxide generating system. Apoptosis, a highly organized physiological mechanism to eliminate injured or abnormal cells, is also implicated in multistage carcinogenesis. It was observed that TOF and ethyl acetate extracts suppressed growth and proliferation of L1210 cells derived from murine lymphoblastic leukaemia. Morphological features of treated cells and characteristic DNA fragmentation revealed that the cytotoxicity was due to induction of apoptosis. This study confirms that TOF and ethyl acetate extracts of C.rotundus possess antibacterial and antioxidant properties and provoke DNA fragmentation, a sign of induction of apoptosis. These results were correlated with chemical composition of the tested extracts.
对莎草科植物香附子块茎提取物的体外抗菌、抗氧化、细胞毒性和凋亡活性进行了研究。评估了不同提取物对五种细菌参考菌株的抗菌活性。总寡聚黄酮（TOFs）提取物和乙酸乙酯提取物对肠炎沙门氏菌、金黄色葡萄球菌和粪肠球菌有显著抑制作用。除抗菌活性外，相同提取物在非酶超氧化物生成系统中还表现出显著抑制超氧阴离子还原硝基蓝四氮唑的能力。凋亡是一种高度有序的生理机制，用于清除受损或异常细胞，也与多阶段致癌作用有关。观察到TOF提取物和乙酸乙酯提取物抑制了源自小鼠淋巴细胞白血病的L1210细胞的生长和增殖。处理后细胞的形态特征和特征性DNA片段化表明，细胞毒性是由凋亡诱导引起的。本研究证实，香附子的TOF提取物和乙酸乙酯提取物具有抗菌和抗氧化特性，并引发DNA片段化，这是凋亡诱导的标志。这些结果与受试提取物的化学成分相关。
