叶下珠茶对健康受试者的抗氧化作用。

Antioxidant effects of Phyllanthus niruri tea on healthy subjects.

作者信息

Colpo Elisângela, Vilanova Carlos D D A, Pereira Romaiana P, Reetz Luis Gustavo B, Oliveira Liliane, Farias Iria L G, Boligon Aline A, Athayde Margareth L, Rocha João Batista T

机构信息

Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.

Laboratório de Análises Clínicas, Hospital Universitário, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.

出版信息

Asian Pac J Trop Med. 2014 Feb;7(2):113-8. doi: 10.1016/S1995-7645(14)60005-5.

DOI:10.1016/S1995-7645(14)60005-5

PMID:24461523

Abstract

OBJECTIVE

To investigate the potential antioxidant effects of Phyllanthus niruri (P. niruri, Euphorbiaceae) tea on healthy subjects.

METHODS

Five non-smoking, male healthy volunteers, 20 to 31 years old, were enrolled. Each subject was treated twice, following a randomized crossover fashion regarding the ingestion of P. niruri infusion (5 g/750 mL) (tea group) or 750 mL of water (control group). Fasting venous blood samples were collected prior to and at 1, 2 and 4 h after infusion drinking. Samples were tested for plasmatic gallic acid and ascorbic acid levels, erythrocytic catalase and superoxide dismutase activities, and intracellular DCFH fluorescence in granulocytes, monocytes and lymphocytes.

RESULTS

Catalase and superoxide dismutase activities were not altered by tea ingestion. Plasma levels of gallic acid were significantly increased at 1, 2 and 4 h after P. niruri ingestion and plasma ascorbic acid at 1 h after P. niruri ingestion.

CONCLUSIONS

Ingestion of P. niruri tea is associated with a slight increase in antioxidant markers in human blood (ascorbic acid and gallic acid), which may contribute to its pharmacological effects.

摘要

目的

研究叶下珠茶对健康受试者的潜在抗氧化作用。

方法

招募了5名年龄在20至31岁之间的不吸烟男性健康志愿者。每位受试者接受两次治疗，按照随机交叉方式饮用叶下珠浸液（5克/750毫升）（茶组）或750毫升水（对照组）。在饮用浸液前以及饮用后1、2和4小时采集空腹静脉血样本。检测样本中的血浆没食子酸和抗坏血酸水平、红细胞过氧化氢酶和超氧化物歧化酶活性，以及粒细胞、单核细胞和淋巴细胞内的二氯荧光素（DCFH）荧光。