不同干燥方法和溶剂萃取制备的叶下珠提取物的代谢物组成与生物活性之间的关系
Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction.
作者信息
Mediani Ahmed, Abas Faridah, Khatib Alfi, Tan Chin Ping, Ismail Intan Safinar, Shaari Khozirah, Ismail Amin, Lajis N H
机构信息
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
出版信息
Plant Foods Hum Nutr. 2015 Jun;70(2):184-92. doi: 10.1007/s11130-015-0478-5.
The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 and 100%) were compared. The relationships among the antioxidant, α-glucosidase inhibitory activity and metabolite levels of the extracts were evaluated using partial least-square analysis (PLS). The solvent selectivity was assessed based on the phytochemical constituents present in the extract and their concentrations quantitatively analyzed using high performance liquid chromatography. The freeze-dried P. niruri samples that were extracted with the mixture of ethanol or methanol with low ratio of water showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different ratio and water extracts demonstrated that phenolic acids (chlorogenic acid and ellagic acid) and flavonoids were highly linked to strong α-glucosidase inhibitory and antioxidant activities.
该研究调查了经过三种干燥处理（空气干燥、冷冻干燥和烘箱干燥）后，叶下珠的代谢物、抗氧化和α-葡萄糖苷酶抑制活性的变化。比较了水提取物以及使用不同乙醇和甲醇溶剂比例（50%、70%、80%和100%）获得的提取物。使用偏最小二乘法分析（PLS）评估提取物的抗氧化、α-葡萄糖苷酶抑制活性与代谢物水平之间的关系。基于提取物中存在的植物化学成分评估溶剂选择性，并使用高效液相色谱法定量分析其浓度。与其他提取物相比，用低水比例的乙醇或甲醇混合物提取的冷冻干燥叶下珠样品显示出更高的生物活性值。不同比例乙醇提取物和水提取物的PLS结果表明，酚酸（绿原酸和鞣花酸）和黄酮类化合物与较强的α-葡萄糖苷酶抑制和抗氧化活性高度相关。
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