Effect of cutting or crushing the rat sciatic nerve on synthesis of substance P by isolated L5 dorsal root ganglia.

Following cutting or crushing the rat sciatic nerve, synthesis of substance P by L5 dorsal root ganglia in vitro was reduced respectively to 20 and 40 per cent of control values. By day 64 the ability to synthesise substance P had been fully restored in crushed neurones but in cut neurones remained at a low level. We conclude that substance P synthesis is a more sensitive index of the effect of nerve injury than is substance P content and further that regenerating axons are able to support substance P synthesis before they reach their target tissue.