Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Biochem Biophys Res Commun. 2014 Feb 7;444(2):230-4. doi: 10.1016/j.bbrc.2014.01.061. Epub 2014 Jan 22.
MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3'-untranslated region (3'-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.
微小 RNA（miRNA）在癌症的发生和发展中发挥着重要作用。在这里，我们研究了 miR-17 在骨肉瘤（OS）进展和转移中的作用。miR-17 在 OS 组织和细胞系中经常增加。在 OS 细胞系中抑制 miR-17 可显著抑制细胞增殖、迁移和侵袭。磷酸酶和张力蛋白同源物（PTEN）被鉴定为 miR-17 的靶标，并且 miR-17 通过直接结合其 3'非翻译区（3'UTR）抑制 PTEN 的表达。miR-17 的表达与 OS 组织中的 PTEN 呈负相关。总之，这些发现表明 miR-17 作为一种致癌 miRNA，可能有助于 OS 的进展和转移，提示 miR-17 可能成为 OS 的一种潜在的新型诊断和治疗靶点。
