1. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Bio-X Center, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People's Republic of China.
2. Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders of Ministry of Education, Bio-X Center, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People's Republic of China.
Theranostics. 2014 Jan 5;4(2):154-62. doi: 10.7150/thno.7560. eCollection 2014.
Successful development of novel electrochemical biosensing interface for ultrasensitive detection of volatile biomarkers of gastric cancer cells is a challenging task. Herein we reported to screen out novel volatile biomarkers associated with gastric cancer cells and develop a novel Au-Ag alloy composites-coated MWCNTs as sensing interface for ultrasensitive detection of volatile biomarkers. MGC-803 gastric cancer cells and GES-1 gastric mucous cells were cultured in serum-free media. The sample preparation approaches and HS-SPME conditions were optimized for screening volatile biomarkers. Volatiles emitted from the headspace of the cells/medium culture were identified using GC-MS. The Au-Ag nanoparticles-coated multiwalled carbon nanotubes were prepared as a sensing interface for detection of volatile biomarkers. Results showed that eight different volatile metabolites were screened out between MGC-803 cells and GES-1 cells. Two compounds such as 3-octanone and butanone were specifically present in the headspace of the MGC-803 cells. Three volatiles such as 4-isopropoxybutanol, nonanol and 4-butoxy 1-butanol coexisted in the headspace of both the MGC-803 cells and the GES-1 cells, their concentrations in the headspace of the GES-1cells were markedly higher than those in the MGC-803 cells, three volatiles such as formic acid propyl ester, 1.4-butanediol and 2, 6, 11-trimethyl dodecane solely existed in the headspace of the GES-1 cells. The nanocomposites of MWNTs loaded with Au-Ag nanoparticles were prepared as a electrochemical sensing interface for detection of two volatile biomarkers, cyclic voltammetry studies showed that the fabricated sensor could detect 3-octanone in the range of 0~0.0025% (v/v) and with a detection limitation of 0.3 ppb, could detect butanone in the range of 0 ~ 0.055% (v/v), and with a detection limitation of 0.5 ppb, and exhibited good selectivity. The novel electrochemical biosensor combined with volatile biomarkers of gastric cancer owns great potential in applications such as early diagnosis and the prognosis of gastric cancer in near future.
成功开发用于超灵敏检测胃癌细胞挥发性生物标志物的新型电化学生物传感界面是一项具有挑战性的任务。在此，我们报告筛选出与胃癌细胞相关的新型挥发性生物标志物，并开发了一种新型 Au-Ag 合金复合材料涂层 MWCNTs 作为传感界面，用于超灵敏检测挥发性生物标志物。在无血清培养基中培养 MGC-803 胃癌细胞和 GES-1 胃黏膜细胞。优化了样品制备方法和 HS-SPME 条件，以筛选挥发性生物标志物。使用 GC-MS 鉴定细胞/培养基培养物顶空释放的挥发物。制备 Au-Ag 纳米粒子涂层多壁碳纳米管作为检测挥发性生物标志物的传感界面。结果表明，在 MGC-803 细胞和 GES-1 细胞之间筛选出 8 种不同的挥发性代谢物。两种化合物，即 3-辛酮和丁酮，特异性存在于 MGC-803 细胞的顶空中。三种挥发性物质，即 4-异丙氧基-1-丁醇、壬醇和 4-丁氧基-1-丁醇，共存于 MGC-803 细胞和 GES-1 细胞的顶空中，它们在 GES-1 细胞顶空中的浓度明显高于 MGC-803 细胞，三种挥发性物质，即丙酸丙酯、1,4-丁二醇和 2,6,11-三甲基十二烷，仅存在于 GES-1 细胞的顶空中。MWNTs 负载 Au-Ag 纳米粒子的纳米复合材料作为电化学传感界面用于检测两种挥发性生物标志物，循环伏安法研究表明，所制备的传感器可以在 00.0025%（v/v）范围内检测 3-辛酮，检测限为 0.3 ppb，可在 00.055%（v/v）范围内检测丁酮，检测限为 0.5 ppb，具有良好的选择性。新型电化学生物传感器与胃癌挥发性生物标志物相结合，在胃癌的早期诊断和预后等方面具有广阔的应用前景。
