Ozeki T, Mizuno S, Sanefuzi H, Tanaka N, Fujiwara H, Masuda H, Funakoshi K
3rd Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Br J Exp Pathol. 1987 Oct;68(5):605-12.
Hepatitis B (HB) core particle, in biopsied liver specimens from patients with chronic active hepatitis (type B) were stained with immunoperoxidase and chromotrope aniline blue (a non-immunological method) and the following results were obtained. HB core particles were more clearly stained with chromotrope aniline blue than with immunoperoxidase. Numerous HB core particles were seen not only in the nuclei of hepatocytes but also in the cytoplasm in HBeAg seropositive patients with severe chronic active hepatitis. DNAase digestion indicated that the HB core particles which stained with immunoperoxidase and chromotrope aniline blue contained DNA. Small amounts of HB core particles were seen in both the nuclei and cytoplasm of all anti-HBe seropositive patients with either moderate or severe chronic active hepatitis.
对慢性活动性乙型肝炎患者活检肝组织标本中的乙肝（HB）核心颗粒，采用免疫过氧化物酶和变色酸苯胺蓝（一种非免疫方法）进行染色，得到以下结果。与免疫过氧化物酶相比，变色酸苯胺蓝对HB核心颗粒的染色更清晰。在HBeAg血清阳性的重度慢性活动性肝炎患者中，不仅在肝细胞细胞核中可见大量HB核心颗粒，在细胞质中也可见到。DNA酶消化表明，经免疫过氧化物酶和变色酸苯胺蓝染色的HB核心颗粒含有DNA。在所有抗HBe血清阳性的中度或重度慢性活动性肝炎患者的细胞核和细胞质中均可见少量HB核心颗粒。
