Bianchi L, Gudat F
Lab Invest. 1976 Jul;35(1):1-5.
In confirmation of earlier descriptions by Huang et al. (Huang S-N, Millman I, O'Connell A, Aronoff A, Gault H, Blumberg BS: Am J Pathol 67: 453, 1972) nuclear eosinophilic inclusions due to excess HBcAg particles have been identified in cases of chronic hepatitis B virus infection. As the euchromatin space of affected nuclei is "sanded" by numerous core particles with concomitant dissolution of the chromatin network, spiky, finely granular, and eosinophilic inclusions without a limiting membrane become visible in hematoxylin and eosin-stained paraffin sections. These HBcAg inclusions stain greyish pink with chromotrope aniline blue and are negative for orcein, the periodic acid-Schiff reaction, and the Feulgen reaction for DNA. Sanded nuclei were detected, although not always and only few in number, exclusively in HBAg-positive patients when a focal (as in chronic aggressive hepatitis) or a generalized core formation (as in immunosuppressed kidney transplant recipients) could be demonstrated by electron microscopy or immunofluorescence. Therefore, the positive finding of sanded nuclei in a persistent hepatitis B virus infection indicates an excessive core formation the extent of which should be verified by specific methods.
正如黄等人（Huang S-N, Millman I, O'Connell A, Aronoff A, Gault H, Blumberg BS: Am J Pathol 67: 453, 1972）早期描述所证实的，在慢性乙型肝炎病毒感染病例中已发现由于过量乙肝核心抗原（HBcAg）颗粒导致的核嗜酸性包涵体。由于受影响细胞核的常染色质空间被大量核心颗粒“磨砂”，同时染色质网络溶解，在苏木精和伊红染色的石蜡切片中可见无界膜的尖刺状、细颗粒状和嗜酸性包涵体。这些HBcAg包涵体用变色苯胺蓝染成灰粉色，对orcein、过碘酸希夫反应和DNA的福尔根反应呈阴性。当通过电子显微镜或免疫荧光证实有局灶性（如在慢性侵袭性肝炎中）或全身性核心形成（如在免疫抑制的肾移植受者中）时，仅在HBAg阳性患者中检测到磨砂核，尽管并非总是如此且数量很少。因此，在持续性乙型肝炎病毒感染中磨砂核的阳性发现表明核心形成过多，其程度应通过特定方法进行验证。
